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PMID: 32708160 Published · epublish English Journal Article

ATG4 Mediated Psm ES4326/AvrRpt2-Induced Autophagy Dependent on Salicylic Acid in Arabidopsis Thaliana.

International journal of molecular sciences ·Vol. 21 ·No. 14 ·2020-07-21

Gong W, Li B, Zhang B, Chen W

Abstract

Psm ES4326/AvrRpt2 (AvrRpt2) was widely used as the reaction system of hypersensitive response (HR) in Arabidopsis. The study showed that in npr1 (GFP-ATG8a), AvrRpt2 was more effective at inducing the production of autophagosome and autophagy flux than that in GFP-ATG8a. The mRNA expression of ATG1, ATG6 and ATG8a were more in npr1 during the early HR. Based on transcriptome data analysis, enhanced disease susceptibility 1 (EDS1) was up-regulated in wild-type (WT) but was not induced in atg4a4b (ATG4 deletion mutant) during AvrRpt2 infection. Compared with WT, atg4a4b had higher expression of salicylic acid glucosyltransferase 1 (SGT1) and isochorismate synthase 1 (ICS1); but less salicylic acid (SA) in normal condition and the same level of free SA during AvrRpt2 infection. These results suggested that the consumption of free SA should be occurred in atg4a4b. AvrRpt2 may trigger the activation of Toll/Interleukin-1 receptor (TIR)-nucleotide binding site (NB)-leucine rich repeat (LRR)-TIR-NB-LRR-to induce autophagy via EDS1, which was inhibited by nonexpressor of PR genes 1 (NPR1). Moreover, high expression of NPR3 in atg4a4b may accelerate the degradation of NPR1 during AvrRpt2 infection.

Keywords
autophagy enhanced disease susceptibility (EDS1) nonexpressor of PR genes 1 (NPR1) salicylic acid (SA)
MeSH Terms
Arabidopsis/genetics,immunology,metabolism,microbiology Arabidopsis Proteins/genetics,metabolism Autophagy/genetics,immunology Autophagy-Related Protein 8 Family/genetics,metabolism Autophagy-Related Proteins/genetics,metabolism Bacterial Proteins/immunology,metabolism Beclin-1/genetics,metabolism Cysteine Proteases/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Glucosyltransferases/genetics,metabolism Host-Pathogen Interactions/genetics,immunology Intramolecular Transferases/genetics,metabolism Membrane Proteins/genetics,metabolism Mitochondrial Proteins/genetics,metabolism Plant Diseases/immunology,microbiology Plants, Genetically Modified Pseudomonas syringae/metabolism,pathogenicity RNA-Seq Salicylic Acid/metabolism
Chemicals
AT3G51790 protein, Arabidopsis ATG8 protein, Arabidopsis Arabidopsis Proteins Autophagy-Related Protein 8 Family Autophagy-Related Proteins Bacterial Proteins Beclin-1 DNA-Binding Proteins EDS1 protein, Arabidopsis Membrane Proteins Mitochondrial Proteins NPR1 protein, Arabidopsis avrRpt2 protein, Pseudomonas syringae beclin 1 protein, Arabidopsis Glucosyltransferases SGT1 protein, Arabidopsis Atg4 protein, Arabidopsis Cysteine Proteases Intramolecular Transferases isochorismate synthase Salicylic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gong Wenjun
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. | Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Li Bingcong
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. | Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Zhang Baihong
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. | Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Chen Wenli ORCID
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. | Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
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Article Info
Journal
International journal of molecular sciences
Abbr.
Int J Mol Sci
ISSN
1422-0067
DOI
10.3390/ijms21145147
Published
2020-07-21
Epub
2020-00-21
Language
English
Region
Switzerland
NLM ID
101092791
PMCID
PMC7404177
Subset
IM
Grants
China Normal University, and a grant from the science and technology project of Guangzhou · No.201805010002
the National Natural Science Foundation of China · 31570256
External Links
PubMed source DOI: 10.3390/ijms21145147 PMC Full Text View journal details
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