Home LiteratureArticle Details
PMID: 3275623 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of Salmonella typhimurium cys genes by transduction with a library of recombinant plasmids packaged in bacteriophage P22HT capsids.

Journal of bacteriology ·Vol. 170 ·No. 1 ·1988-01-00 ·Pages 42-7

Monroe RS, Kredich NM

Abstract

We have prepared a library of Salmonella typhimurium genomic fragments cloned in pBR322 and packaged in P22HT capsids. Plasmids carrying 24 of 26 specific genes searched for were isolated by transduction at frequencies of 1 to 344 per 10(6) plasmid transductants. All 11 known genes of the cysteine regulon were isolated from this library, including cysK, which we had previously been unable to clone in a recombinant plasmid with an Escherichia coli host. This library provides a simple and rapid method for isolating most S. typhimurium genes by using S. typhimurium itself as a host and should be particularly useful for cloning genes that might be deleterious to E. coli.

MeSH Terms
Bacteriophages/genetics Capsid/genetics Cloning, Molecular Cysteine/biosynthesis,genetics Genes, Bacterial Plasmids Salmonella typhimurium/genetics Transduction, Genetic Transformation, Bacterial
Chemicals
Cysteine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Monroe R S
Howard Hughes Medical Institute, Duke University, Durham, North Carolina.
Kredich N M
References (23)
23 references, click to expand
  1. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
  2. Formation of phage receptors induced by galactose in a galactose-sensitive mutant of Salmonella.
    Virology. 1960 Jun;11:508-10 PMID: 13825591
  3. Regulation of L-cysteine biosynthesis in Salmonella typhimurium. I. Effects of growth of varying sulfur sources and O-acetyl-L-serine on gene expression.
    J Biol Chem. 1971 Jun 10;246(11):3474-84 PMID: 4931306
  4. Deletion mapping of the genes coding for HPr and enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system in Salmonella typhimurium.
    J Bacteriol. 1972 Oct;112(1):17-29 PMID: 4562394
  5. Phage P22-mutants with increased or decreased transduction abilities.
    Mol Gen Genet. 1972;119(1):75-88 PMID: 4564719
  6. A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.
    Biochim Biophys Acta. 1973 Apr 11;299(4):516-20 PMID: 4575181
  7. The structural gene for O-acetylserine sulfhydrylase A in Salmonella typhimurium. Identity with the trzA locus.
    J Biol Chem. 1974 Feb 10;249(3):867-72 PMID: 4204554
  8. Evidence that HT mutant strains of bacteriophage P22 retain an altered form of substrate specificity in the formation of transducing particles in Salmonella typhimurium.
    Genet Res. 1976 Apr;27(2):315-22 PMID: 776744
  9. EcoRI analysis of bacteriophage P22 DNA packaging.
    J Mol Biol. 1978 Jan 25;118(3):365-88 PMID: 344888
  10. Regulation of O-acetylserine sulfhydrylase B by L-cysteine in Salmonella typhimurium.
    J Bacteriol. 1979 Oct;140(1):141-6 PMID: 387718
  11. Transfer of chimeric plasmids among Salmonella typhimurium strains by P22 transduction.
    J Bacteriol. 1982 Mar;149(3):985-94 PMID: 6277858
  12. Packaging signals for phage P22 on the chromosome of Salmonella typhimurium.
    Mol Gen Genet. 1982;187(3):516-8 PMID: 6757664
  13. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  14. Cloning and physical mapping of the cysB region of Salmonella typhimurium.
    J Bacteriol. 1983 Aug;155(2):578-85 PMID: 6307971
  15. Salmonella typhimurium LT2 strains which are r- m+ for all three chromosomally located systems of DNA restriction and modification.
    J Bacteriol. 1983 Oct;156(1):471-4 PMID: 6352690
  16. Linkage map of Salmonella typhimurium, Edition VI.
    Microbiol Rev. 1983 Sep;47(3):410-53 PMID: 6314110
  17. Location on the Escherichia coli genome of a gene specifying O-acetylserine (thiol)-lyase.
    J Gen Microbiol. 1984 Mar;130(3):673-85 PMID: 6374031
  18. Selective transduction of recombinant plasmids with cloned pac sites by Salmonella phage P22.
    Mol Gen Genet. 1984;196(1):123-8 PMID: 6592417
  19. Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains.
    J Bacteriol. 1985 Jan;161(1):442-5 PMID: 3881397
  20. Cloning of a restriction-modification system from Proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in Escherichia coli.
    J Bacteriol. 1985 Nov;164(2):501-9 PMID: 2997113
  21. Cloning and characterization of the cysAMK region of Salmonella typhimurium.
    J Bacteriol. 1986 Oct;168(1):322-7 PMID: 3531173
  22. Bacterial transduction.
    J Cell Physiol Suppl. 1955 May;45(Suppl. 2):23-49 PMID: 13242624
  23. GENETIC STRUCTURE OF THE CYST REGION OF THE SALMONELLA GENOME.
    Genetics. 1963 Aug;48:997-1009 PMID: 14054326
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.1.42-47.1988
Published
1988-01-00
Pages
42-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210603
Subset
IM
Grants
NIDDK NIH HHS · DK-12828 · United States
NIGMS NIH HHS · GM-07184 · United States
External Links
PubMed source DOI: 10.1128/jb.170.1.42-47.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2