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PMID: 3531173 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of the cysAMK region of Salmonella typhimurium.

Journal of bacteriology ·Vol. 168 ·No. 1 ·1986-10-00 ·Pages 322-7

Hulanicka MD, Garrett C, Jagura-Burdzy G, Kredich NM

Abstract

A total of 30 kilobases of DNA comprising the cysAMK region of S. typhimurium was cloned as a series of fragments in phage lambda 1059. The genetic organization of this region was established through studies of gene expression from fragments subcloned in pBR322 and from blot hydridization analyses of restriction sites in chromosomal DNA from multisite deletion strains. The results give a gene order of cysA-cysM-crr-ptsl-ptsH-cysK over a distance of approximately 12 kilobases. cysM and cysA have been cloned and expressed in pBR322; attempts to obtain stable pBR322 derivatives carrying cysK were unsuccessful.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular Cysteine/biosynthesis Cysteine Synthase/genetics Genes, Bacterial Lyases/genetics Promoter Regions, Genetic Salmonella typhimurium/genetics,metabolism
Chemicals
Cysteine Synthase Lyases Cysteine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hulanicka M D
Garrett C
Jagura-Burdzy G
Kredich N M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.168.1.322-327.1986
Published
1986-10-00
Pages
322-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213454
Subset
IM
Grants
NIADDK NIH HHS · AM-12828 · United States
External Links
PubMed source DOI: 10.1128/jb.168.1.322-327.1986 PMC Full Text View journal details
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