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PMID: 387741 Published · ppublish English Journal Article

Deoxyribonucleic acid adenine and cytosine methylation in Salmonella typhimurium and Salmonella typhi.

Journal of bacteriology ·Vol. 140 ·No. 2 ·1979-11-00 ·Pages 574-9

Gómez-Eichelmann MC

Abstract

The methylations of adenine in the sequence -GATC- and of the second cytosine in the sequence - [Formula: see text] - were studied in Salmonella typhimurium and in Salmonella typhi. The study was carried out by using endonucleases which restrict the plasmid pBR322 by cleavage at the sequences -GATC- (DpnI and MboI) and - [Formula: see text] - (EcoRII). The restriction patterns obtained for this plasmid isolated from transformed S. typhimurium and S. typhi were compared with those of pBR322 isolated from Escherichia coli K-12. In E. coli K-12, adenines at the sequence -GATC- and the second cytosines at - [Formula: see text] - are met hylated by enzymes coded for by the genes dam and dem, respectively. From comparison of the restriction patterns obtained, it is concluded that S. typhimurium and S. typhi contain genes responsible for deoxyribonucleic acid methylation equivalent to E. coli K-12 genes dam and dcm.

MeSH Terms
Adenine/metabolism Base Sequence Cytosine/metabolism DNA Restriction Enzymes DNA, Bacterial/metabolism Genes Methylation Methyltransferases/genetics Salmonella/genetics Salmonella typhi/metabolism Salmonella typhimurium/metabolism
Chemicals
DNA, Bacterial Cytosine Methyltransferases DNA Restriction Enzymes Adenine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Gómez-Eichelmann M C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.140.2.574-579.1979
Published
1979-11-00
Pages
574-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216684
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.140.2.574-579.1979 PMC Full Text View journal details
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