Subcellular localization of two porphyrin-synthesis enzymes in Pisum sativum (pea) and Arum (cuckoo-pint) species.

The Biochemical journal ·Vol. 249 ·No. 2 ·1988-01-15 ·Pages 423-8

Smith AG

Abstract

The subcellular location of the two porphyrin-synthesis enzymes 5-aminolaevulinate dehydratase (ALAD) and porphobilinogen deaminase (PBGD) was investigated in Pisum sativum (pea) leaves and spadices of Arum (cuckoo-pint). Throughout the tissue-fractionation procedures the distribution of the two enzymes paralleled that of the plastid marker enzyme (ADP-glucose pyrophosphorylase), even in Arum, a tissue where the synthesis of non-plastid haem is predominant. The distribution of cytosolic marker enzyme (lactate dehydrogenase) was significantly different from that of ALAD and PBGD and, although purified mitochondria from both species had some residual activity, this was always less than contaminating plastid marker enzyme. The results suggest that ALAD and PBGD are exclusively plastid enzymes. The significance of this for the role of plastids in cellular porphyrin synthesis is discussed.

MeSH Terms

Alcohol Oxidoreductases/metabolism Ammonia-Lyases/metabolism Centrifugation, Density Gradient Fabaceae/enzymology Hydroxymethylbilane Synthase/metabolism Hydroxypyruvate Reductase Malate Dehydrogenase/metabolism Plants/enzymology Plants, Medicinal Porphobilinogen Synthase/metabolism Porphyrins/biosynthesis Subcellular Fractions/enzymology Trypsin/pharmacology

Chemicals

Porphyrins Alcohol Oxidoreductases Malate Dehydrogenase Hydroxypyruvate Reductase Hydroxymethylbilane Synthase Trypsin Porphobilinogen Synthase Ammonia-Lyases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Smith A G

Department of Botany, University of Cambridge, U.K.

References (11)

11 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2490423

Published

1988-01-15

Pages

423-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1148720

Subset

External Links

PubMed source DOI: 10.1042/bj2490423 PMC Full Text View journal details

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Subcellular localization of two porphyrin-synthesis enzymes in Pisum sativum (pea) and Arum (cuckoo-pint) species.

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