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PMID: 3277952 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene.

Journal of bacteriology ·Vol. 170 ·No. 3 ·1988-03-00 ·Pages 1268-74

Coleman J, Raetz CR

Abstract

The min 4 region of the Escherichia coli genome contains genes (lpxA and lpxB) that encode proteins involved in lipid A biosynthesis. We have determined the sequence of 1,350 base pairs of DNA upstream of the lpxB gene. This fragment of DNA contains the complete coding sequence for the 28.0-kilodalton lpxA gene product and an upstream open reading frame capable of encoding a 17-kilodalton protein (ORF17). In addition there appears to be an additional open reading frame (ORF?) immediately upstream of ORF17. The initiation codon for lpxA is a GUG codon, and the start codon for ORF17 is apparently a UUG codon. The start and stop codons overlap between ORF? and ORF17, ORF17 and lpxA, and lpxA and lpxB. This overlap is suggestive of translational coupling and argues that the genes are cotranscribed. Crowell et al. (D.N. Crowell, W.S. Reznikoff, and C.R.H. Raetz, J. Bacteriol. 169:5727-5734, 1987) and Tomasiewicz and McHenry (H.G. Tomasiewicz and C.S. McHenry, J. Bacteriol. 169:5735-5744, 1987) have demonstrated that there are three similarly overlapping coding regions downstream of lpxB including dnaE, suggesting the existence of a complex operon of at least seven genes: 5'-ORF?-ORF17-lpxA-lpxB-ORF23-dnaE-ORF37-3 '.

MeSH Terms
Acyltransferases/genetics Amino Acid Sequence Base Sequence Codon Escherichia coli/genetics Genes, Bacterial Lipid A/biosynthesis Molecular Sequence Data Molecular Weight Peptide Chain Initiation, Translational
Chemicals
Codon Lipid A Acyltransferases acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine acyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Coleman J
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Raetz C R
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.3.1268-1274.1988
Published
1988-03-00
Pages
1268-74
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210902
Subset
IM
Grants
NIDDK NIH HHS · DK-19551 · United States
Databases
GENBANK
M18265, M18266, M19334
External Links
PubMed source DOI: 10.1128/jb.170.3.1268-1274.1988 PMC Full Text View journal details
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