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PMID: 3279415 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Three binding sites for AraC protein are required for autoregulation of araC in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 6 ·1988-03-00 ·Pages 1749-53

Hamilton EP, Lee N

Abstract

Three binding sites for AraC protein were shown to be required for the autoregulation of araC: araI1, araO1, and araO2. Selective inactivation of AraC-binding sites on the DNA demonstrated that araO1 and araO2 are required in vivo to produce repression of araC in the presence of arabinose, whereas araI1 and araO2 are required in its absence. We found that the low-affinity site araO2 is essential for araC autoregulation; araO1 and araI1 provide high-affinity AraC-binding sites, which allow cooperative binding at araO2. Profound effects on the araBAD promoter and the araC promoter are produced by ligand-induced changes in AraC occupancy of functional sites on the DNA. We suggest that AraC exerts its multiplicity of controls through two alternative states of cooperative interactions with DNA and we illustrate this with a model. This model presents our interpretations of activation and repression of the araBAD operon and the autoregulation of the araC gene.

MeSH Terms
AraC Transcription Factor Bacterial Proteins Base Sequence Binding Sites DNA, Bacterial/metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics Escherichia coli Proteins Homeostasis Molecular Sequence Data Mutation Repressor Proteins/genetics Transcription Factors/genetics
Chemicals
AraC Transcription Factor AraC protein, E coli Bacterial Proteins DNA, Bacterial Escherichia coli Proteins Repressor Proteins Transcription Factors DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hamilton E P
Department of Biological Sciences, University of California, Santa Barbara 93106.
Lee N
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.6.1749
Published
1988-03-00
Pages
1749-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279856
Subset
IM
Grants
NIGMS NIH HHS · 5 RO1 GM14652-19 · United States
NCRR NIH HHS · 507 RR07099-20 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.6.1749 PMC Full Text View journal details
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