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PMID: 3290201 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Colicin E8, a DNase which indicates an evolutionary relationship between colicins E2 and E3.

Journal of bacteriology ·Vol. 170 ·No. 7 ·1988-07-00 ·Pages 3237-42

Toba M, Masaki H, Ohta T

Abstract

Colicin E8-J and its immunity protein were characterized with regard to their activities and gene structures. Colicin E8 is a complex of proteins A and B; protein A (the naked E8) exhibits an apparently nonspecific DNase activity that is inhibited by protein B (the immunity protein), as in the case of colicin E2. The nucleotide sequence of the downstream half of the colicin operon of ColE8-J was determined to be highly homologous to that of ColE2-P9, with the exception of the hot spot region of the 3'-terminal segment of the colicin gene and the adjacent immunity gene. The immE2-like gene of ColE3-CA38 was, as assumed previously, extensively homologous to the immE8 gene of ColE8-J, and thus, ColE8-J was shown to be situated between ColE2-P9 and ColE3-CA38 in the evolution of the E-group Col plasmids.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,isolation & purification Bacteriocin Plasmids Base Sequence Biological Evolution Colicins/antagonists & inhibitors,genetics,isolation & purification DNA, Bacterial/genetics Deoxyribonucleases/genetics,isolation & purification Escherichia coli/enzymology,genetics Escherichia coli Proteins Genes, Bacterial Molecular Sequence Data Operon Plasmids Sequence Homology, Nucleic Acid
Chemicals
Bacterial Proteins Colicins DNA, Bacterial Escherichia coli Proteins ceiC protein, E coli colicin immunity proteins immE8 protein, E coli Deoxyribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Toba M
Department of Agricultural Chemistry, University of Tokyo, Japan.
Masaki H
Ohta T
References (28)
28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.7.3237-3242.1988
Published
1988-07-00
Pages
3237-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211275
Subset
IM
Databases
GENBANK
M21404
External Links
PubMed source DOI: 10.1128/jb.170.7.3237-3242.1988 PMC Full Text View journal details
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