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PMID: 3293998 Published · ppublish English Journal Article

Expression of the FGF-related proto-oncogene int-2 during gastrulation and neurulation in the mouse.

The EMBO journal ·Vol. 7 ·No. 3 ·1988-03-00 ·Pages 691-5

Wilkinson DG, Peters G, Dickson C, McMahon AP

Abstract

The proto-oncogene int-2 has been implicated in the formation of mouse mammary-tumour-virus-induced mammary tumours. Analysis of the predicted coding sequence indicates that int-2 is a member of the fibroblast growth factor family. Previous studies using Northern blot analysis suggested that normal expression of int-2 may be confined to extra-embryonic endoderm lineages of embryonic stages of mouse development. We have used in situ hybridization and Northern blot analysis to examine directly int-2 expression in embryo stem cells and in the developing embryo from early gastrulation to midsomite stages. Complex patterns of accumulation of int-2 RNA were observed in embryonic and extra-embryonic tissues. The data suggest multiple roles for int-2 in development which may include migration of early mesoderm cells and induction of the otocyst.

MeSH Terms
Animals Brain/embryology,metabolism Embryo, Mammalian/metabolism Endoderm/metabolism Fibroblast Growth Factors/physiology Gene Expression Regulation Mesoderm/metabolism Mice Mice, Inbred CBA Nucleic Acid Hybridization Proto-Oncogene Proteins/metabolism,physiology RNA, Messenger/metabolism,physiology
Chemicals
Proto-Oncogene Proteins RNA, Messenger Fibroblast Growth Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wilkinson D G
Laboratory of Developmental Biochemistry, National Institute for Medical Research, Mill Hill, London, UK.
Peters G
Dickson C
McMahon A P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-03-00
Pages
691-5
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454374
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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