Sequence, topography and protein coding potential of mouse int-2: a putative oncogene activated by mouse mammary tumour virus.

The EMBO journal ·Vol. 5 ·No. 5 ·1986-05-00 ·Pages 919-24

Moore R, Casey G, Brookes S, Dixon M, Peters G, Dickson C

Abstract

A major proportion of carcinomas induced by mouse mammary tumour virus (MMTV) show evidence for proviral activation of a cellular gene, int-2, on chromosome 7. The sequence of 7869 bp of DNA spanning the transcription unit of int-2 was determined and compared with that of a series of int-2-specific cDNA clones derived from mammary tumour RNA. The predicted positions of intron-exon boundaries, established by alignment of cDNA and chromosomal DNA sequences, indicate that the gene comprises at least three exons. An open reading frame capable of encoding a protein of 245 amino acids with an estimated mol. wt of 27 kd, is flanked by substantial non-coding segments at both 5' and 3' ends. Comparison of the chromosomal DNA sequence and the predicted amino acid sequence with available data-bases has revealed no homology to other known genes. These results are discussed in relation to the status of int-2 as a candidate proto-oncogene.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cell Transformation, Neoplastic Chromosome Mapping Cloning, Molecular DNA/analysis Genes Mammary Tumor Virus, Mouse/genetics Mice Neoplasm Proteins/genetics Oncogenes RNA, Neoplasm/isolation & purification Transcription, Genetic

Chemicals

Neoplasm Proteins RNA, Neoplasm DNA

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Moore R

Casey G

Brookes S

Dixon M

Peters G

Dickson C

References (26)

26 references, click to expand

Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
Polyadenylation of the Xenopus beta 1 globin mRNA at a downstream minor site in the absence of the major site and utilization of an AAUACA polyadenylation signal.
EMBO J. 1985 Jan;4(1):205-11 PMID: 2862026
A new computer method for the storage and manipulation of DNA gel reading data.
Nucleic Acids Res. 1980 Aug 25;8(16):3673-94 PMID: 7433103
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
Nucleotide sequences at host-proviral junctions for mouse mammary tumour virus.
Nature. 1981 Jan 22;289(5795):253-8 PMID: 6256658
Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis.
Nature. 1981 Apr 9;290(5806):475-80 PMID: 6261142
On the mechanism of retrovirus-induced avian lymphoid leukosis: deletion and integration of the proviruses.
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3418-22 PMID: 6267589
Specific integration of REV proviruses in avian bursal lymphomas.
Nature. 1981 Dec 10;294(5841):574-6 PMID: 6273749
Multiple arrangements of viral DNA and an activated host oncogene in bursal lymphomas.
Nature. 1982 Jan 21;295(5846):209-14 PMID: 6276760
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
Gene. 1982 Oct;19(3):269-76 PMID: 6295880
Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome.
Cell. 1982 Nov;31(1):99-109 PMID: 6297757
Tumorigenesis by mouse mammary tumor virus: evidence for a common region for provirus integration in mammary tumors.
Cell. 1983 Jun;33(2):369-77 PMID: 6305506
Cellular oncogenes and retroviruses.
Annu Rev Biochem. 1983;52:301-54 PMID: 6351725
A simple and very efficient method for generating cDNA libraries.
Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
Mouse mammary tumor virus integration regions int-1 and int-2 map on different mouse chromosomes.
Mol Cell Biol. 1984 Feb;4(2):375-8 PMID: 6321961
Proviruses are adjacent to c-myc in some murine leukemia virus-induced lymphomas.
Proc Natl Acad Sci U S A. 1984 Apr;81(7):2097-101 PMID: 6326104
Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene.
Proc Natl Acad Sci U S A. 1984 Apr;81(7):2147-51 PMID: 6326107
Murine T lymphomas in which the cellular myc oncogene has been activated by retroviral insertion.
Cell. 1984 May;37(1):113-22 PMID: 6327047
Tumorigenesis by mouse mammary tumor virus: proviral activation of a cellular gene in the common integration region int-2.
Cell. 1984 Jun;37(2):529-36 PMID: 6327073
Compilation of published signal sequences.
Nucleic Acids Res. 1984 Jul 11;12(13):5145-64 PMID: 6379599
HMG CoA reductase: a negatively regulated gene with unusual promoter and 5' untranslated regions.
Cell. 1984 Aug;38(1):275-85 PMID: 6088070
Activation of the c-myb locus by viral insertional mutagenesis in plasmacytoid lymphosarcomas.
Science. 1984 Nov 30;226(4678):1077-80 PMID: 6093260
Involvement of c-myc in MuLV-induced T cell lymphomas in mice: frequency and mechanisms of activation.
EMBO J. 1984 Dec 20;3(13):3215-22 PMID: 6098468
The molecular genetics of cellular oncogenes.
Annu Rev Genet. 1984;18:553-612 PMID: 6397126
c-erbB activation in ALV-induced erythroblastosis: novel RNA processing and promoter insertion result in expression of an amino-truncated EGF receptor.
Cell. 1985 Jul;41(3):719-26 PMID: 2988784
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835

Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1986-05-00

Pages

919-24

Language

English

Region

England

NLM ID

8208664

PMCID

PMC1166883

Subset

Databases

GENBANK

Y00848

External Links

PubMed source PMC Full Text View journal details

Analysis Services

Sequence, topography and protein coding potential of mouse int-2: a putative oncogene activated by mouse mammary tumour virus.

Contact

WeChat Official Account