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PMID: 3294181 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning of the gene encoding streptococcal immunoglobulin A protease and its expression in Escherichia coli.

Infection and immunity ·Vol. 56 ·No. 8 ·1988-08-00 ·Pages 1961-6

Gilbert JV, Plaut AG, Fishman Y, Wright A

Abstract

We have identified and cloned a 6-kilobase-pair segment of chromosomal DNA from Streptococcus sanguis ATCC 10556 that encodes immunoglobulin A (IgA) protease activity when cloned into Escherichia coli. The enzyme specified by the iga gene in plasmid pJG1 accumulates in the periplasm of E. coli MM294 cells and has a substrate specificity for human IgA1 identical to that of native S. sanguis protease. Hybridization experiments with probes from within the encoding DNA showed no detectable homology at the nucleotide sequence level with chromosomal DNA of gram-negative bacteria that excrete IgA protease. Moreover, the S. sanguis iga gene probes showed no detectable hybridization with chromosomal DNA of S. pneumoniae, although the IgA proteases of these two streptococcal species cleaved the identical peptide bond in the human IgA1 heavy-chain hinge region.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Bacterial/genetics Escherichia coli Genes, Bacterial Molecular Sequence Data Peptide Hydrolases/genetics Recombinant Proteins Serine Endopeptidases Streptococcus sanguis/enzymology,genetics
Chemicals
DNA, Bacterial Recombinant Proteins Peptide Hydrolases Serine Endopeptidases IgA-specific serine endopeptidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gilbert J V
Department of Medicine, Tufts-New England Medical Center Hospital, Boston, Massachusetts.
Plaut A G
Fishman Y
Wright A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.8.1961-1966.1988
Published
1988-08-00
Pages
1961-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259508
Subset
IM
Grants
NIAID NIH HHS · AI 20337 · United States
NIDCR NIH HHS · DE 07257 · United States
NIADDK NIH HHS · P30-AM 39428 · United States
External Links
PubMed source DOI: 10.1128/iai.56.8.1961-1966.1988 PMC Full Text View journal details
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