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PMID: 3308453 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Maturation of vacuolar (lysosomal) enzymes in yeast: proteinase yscA and proteinase yscB are catalysts of the processing and activation event of carboxypeptidase yscY.

The EMBO journal ·Vol. 6 ·No. 7 ·1987-07-00 ·Pages 2157-63

Mechler B, Müller H, Wolf DH

Abstract

Studies were performed to unravel the activation and maturation mechanism of vacuolar (lysosomal) proteinases in Saccharomyces cerevisiae. In vivo and in vitro studies show that proteinase yscA and proteinase yscB are involved in the activation and processing event of pro-carboxypeptidase yscY. Processing and activation of pro-carboxypeptidase yscY by proteinase yscA depends on an additional factor contained in the vacuolar fraction. Comparable activation can be mimicked by sodium polyphosphate. Optimum pH for processing by this proteinase yscA-triggered event is 5. The proteinase yscA-triggered maturation process of pro-carboxypeptidase yscY leads to an intermediate mol. wt form of the enzyme which is, however, fully active. Proteinase yscB transfers the intermediate mol. wt form of the original precursor to the apparently authentic, mature and active carboxypeptidase yscY. An activation and maturation scheme is devised.

MeSH Terms
Aspartic Acid Endopeptidases Carboxypeptidases/genetics,metabolism Cathepsin A Endopeptidases/metabolism Enzyme Activation Kinetics Lysosomes/enzymology Mutation Organoids/enzymology Protein Processing, Post-Translational Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Serine Endopeptidases/metabolism Vacuoles/enzymology
Chemicals
Saccharomyces cerevisiae Proteins Carboxypeptidases Endopeptidases Cathepsin A PRC1 protein, S cerevisiae serine carboxypeptidase Serine Endopeptidases yeast proteinase B aspartic proteinase A PEP4 protein, S cerevisiae Aspartic Acid Endopeptidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mechler B
Biochemisches Institut, Universität Freiburg, FRG.
Müller H
Wolf D H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-07-00
Pages
2157-63
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553608
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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