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PMID: 3309127 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Primary structure of lymphocyte function-associated antigen 3 (LFA-3). The ligand of the T lymphocyte CD2 glycoprotein.

The Journal of experimental medicine ·Vol. 166 ·No. 4 ·1987-10-01 ·Pages 923-32

Wallner BP, Frey AZ, Tizard R, Mattaliano RJ, Hession C, Sanders ME, Dustin ML, Springer TA

Abstract

We have isolated the cDNA for human lymphocyte function-associated antigen 3 (LFA-3), the ligand of the T lymphocyte CD2 molecule. The identity of the clones was established by comparison of the deduced amino acid sequence to the LFA-3 NH2-terminal and tryptic peptide sequences. The cDNA defines a mature protein of 222 amino acids that structurally resembles typical membrane-anchored proteins. An extracellular domain with six N-linked glycosylation sites is followed by a hydrophobic putative transmembrane region and a short cytoplasmic domain. The mature glycoprotein is estimated to be 44-68% carbohydrate. Southern blots of human genomic DNA indicate that only one gene codes for human LFA-3. Northern blot analysis demonstrates that the LFA-3 mRNA of 1.3 kb is widely distributed in human tissues and cell lines.

MeSH Terms
Amino Acid Sequence Antigens, Differentiation, T-Lymphocyte/metabolism Antigens, Surface/genetics,metabolism Base Sequence Cloning, Molecular DNA/analysis Humans Lymphocyte Function-Associated Antigen-1 Molecular Sequence Data Peptide Mapping
Chemicals
Antigens, Differentiation, T-Lymphocyte Antigens, Surface Lymphocyte Function-Associated Antigen-1 DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Wallner B P
Biogen Research Corporation, Cambridge, Massachusetts 02142.
Frey A Z
Tizard R
Mattaliano R J
Hession C
Sanders M E
Dustin M L
Springer T A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.166.4.923
Published
1987-10-01
Pages
923-32
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188720
Subset
IM
Grants
NCI NIH HHS · CA-31798 · United States
Databases
GENBANK
Y00636
External Links
PubMed source DOI: 10.1084/jem.166.4.923 PMC Full Text View journal details
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