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PMID: 331321 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Recombinant plasmid that carries part of the nitrogen fixation (nif) gene cluster of Klebsiella pneumoniae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 7 ·1977-07-00 ·Pages 2963-7

Cannon FC, Riedel GE, Ausubel FM

Abstract

We have cloned fragments of the Klebsiella pneumoniae genome that carry part of the his operon and part of the nitrogen fixation (nif) gene cluster on the amplifiable plasmid pMB9. One particular plasmid, pCRA37, complements mutations in the hisD, nifB, and nifF loci. The physical map of pCRA37 as determined by restriction enzyme analysis correlates with the genetic map of the his-nif region as determined previously by phage P1-mediated cotransductional analysis.

MeSH Terms
Chromosome Mapping DNA Restriction Enzymes/metabolism DNA, Bacterial/metabolism DNA, Recombinant/metabolism Genes Genetic Linkage Histidine/metabolism Klebsiella pneumoniae Nitrogen Fixation Operon Plasmids
Chemicals
DNA, Bacterial DNA, Recombinant Histidine DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cannon F C
Riedel G E
Ausubel F M
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.7.2963
Published
1977-07-00
Pages
2963-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431364
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.7.2963 PMC Full Text View journal details
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