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PMID: 3313276 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of the positively acting regulatory gene QUTA from Aspergillus nidulans.

Nucleic acids research ·Vol. 15 ·No. 19 ·1987-10-12 ·Pages 7991-8001

Beri RK, Whittington H, Roberts CF, Hawkins AR

Abstract

The positively acting regulator gene QUTA from Aspergillus nidulans has been identified and located within a cluster of quinic acid utilisation (QUT) genes isolated within a recombinant phage lambda (lambda Q1). The DNA sequence of the QUTA gene reveals a single uninterrupted reading frame coding for a protein of mw 90.416 Kd. The QUTA protein sequence has a protein motif in the form of a putative "DNA finger" that shows strong homology to other such motifs in the GAL4, PPR1, ARGRII, LAC9 and QA1F regulatory gene products of S. cerevisiae, K. lactis and N. crassa. The data presented confirm the view deduced by genetical analysis that the QUTA gene of A. nidulans encodes a protein capable of interacting with QUT specific DNA sequences.

MeSH Terms
Amino Acid Sequence Aspergillus nidulans/genetics Base Sequence Fungal Proteins/genetics Genes, Fungal Genes, Regulator Introns Molecular Sequence Data Quinic Acid/metabolism Sequence Homology, Nucleic Acid
Chemicals
Fungal Proteins Quinic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Beri R K
Department of Genetics, University of Leicester, UK.
Whittington H
Roberts C F
Hawkins A R
References (33)
33 references, click to expand
  1. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  2. Gene organization and regulation in the qa (quinic acid) gene cluster of Neurospora crassa.
    Microbiol Rev. 1985 Sep;49(3):338-58 PMID: 2931582
  3. Analysis of the Kluyveromyces lactis positive regulatory gene LAC9 reveals functional homology to, but sequence divergence from, the Saccharomyces cerevisiae GAL4 gene.
    Nucleic Acids Res. 1986 Oct 10;14(19):7767-81 PMID: 3022234
  4. Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA.
    Nature. 1983 Oct 13-19;305(5935):600-5 PMID: 6194440
  5. Cloning and characterization of the three enzyme structural genes QUTB, QUTC and QUTE from the quinic acid utilization gene cluster in Aspergillus nidulans.
    Curr Genet. 1985;9(4):305-11 PMID: 3916726
  6. Nucleotide sequence of the ARGRII regulatory gene and amino acid sequence homologies between ARGRII PPRI and GAL4 regulatory proteins.
    Eur J Biochem. 1986 May 15;157(1):77-81 PMID: 3709534
  7. Sequence analysis and transformation by the catabolic 3-dehydroquinase (QUTE) gene from Aspergillus nidulans.
    Biochem J. 1986 Dec 1;240(2):481-8 PMID: 2949740
  8. Chemical synthesis of a gene for somatomedin C.
    Nucleic Acids Res. 1985 Apr 25;13(8):2959-77 PMID: 2987870
  9. An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels.
    Anal Biochem. 1983 Jul 1;132(1):14-9 PMID: 6312834
  10. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  11. The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3' termini.
    Nucleic Acids Res. 1985 Feb 25;13(4):1347-68 PMID: 2987822
  12. Simultaneous rapid chemical synthesis of over one hundred oligonucleotides on a microscale.
    EMBO J. 1984 Apr;3(4):801-5 PMID: 16453516
  13. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  14. Linkage of adult alpha- and beta-globin genes in X. laevis and gene duplication by tetraploidization.
    Cell. 1980 Sep;21(2):555-64 PMID: 7407927
  15. Potential metal-binding domains in nucleic acid binding proteins.
    Science. 1986 Apr 25;232(4749):485-7 PMID: 2421409
  16. Nucleotide sequence encoding the biosynthetic dehydroquinase function of the penta-functional arom locus of Aspergillus nidulans.
    Nucleic Acids Res. 1985 Nov 25;13(22):8119-28 PMID: 3906567
  17. The complex Arom locus of Aspergillus nidulans. Evidence for multiple gene fusions and convergent evolution.
    Curr Genet. 1987;11(6-7):491-8 PMID: 2836080
  18. The isolation and nucleotide sequence of the complex AROM locus of Aspergillus nidulans.
    Nucleic Acids Res. 1986 Mar 11;14(5):2201-13 PMID: 3515316
  19. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  20. Protein-DNA recognition.
    Annu Rev Biochem. 1984;53:293-321 PMID: 6236744
  21. Identification and isolation of a putative permease gene in the quinic acid utilization (QUT) gene cluster of Aspergillus nidulans.
    Curr Genet. 1987;12(2):135-9 PMID: 2835177
  22. Empirical predictions of protein conformation.
    Annu Rev Biochem. 1978;47:251-76 PMID: 354496
  23. Genetical and biochemical aspects of quinate breakdown in the filamentous fungus Aspergillus nidulans.
    Biochem Genet. 1982 Apr;20(3-4):271-86 PMID: 7049157
  24. Cloning and expression in Escherichia coli K-12 of the biosynthetic dehydroquinase function of the arom cluster gene from the eucaryote, Aspergillus nidulans.
    Mol Gen Genet. 1982;186(1):145-52 PMID: 6287167
  25. Evidence for two control genes regulating expression of the quinic acid utilization (qut) gene cluster in Aspergillus nidulans.
    J Gen Microbiol. 1984 Mar;130(3):567-74 PMID: 6374025
  26. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
    EMBO J. 1985 Jun;4(6):1609-14 PMID: 4040853
  27. Expression of qa-1F activator protein: identification of upstream binding sites in the qa gene cluster and localization of the DNA-binding domain.
    Mol Cell Biol. 1987 Mar;7(3):1256-66 PMID: 2951591
  28. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.
    J Bacteriol. 1969 Jan;97(1):244-9 PMID: 4884815
  29. Yeast regulatory gene PPR1. I. Nucleotide sequence, restriction map and codon usage.
    J Mol Biol. 1984 Dec 5;180(2):239-50 PMID: 6096561
  30. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  31. Analysis of acetate non-utilizing (acu) mutants in Aspergillus nidulans.
    J Gen Microbiol. 1976 Feb;92(2):263-82 PMID: 3622
  32. Development of a high-frequency transforming vector for Aspergillus nidulans.
    Gene. 1985;36(3):321-31 PMID: 3000883
  33. Sequence homology of the yeast regulatory protein ADR1 with Xenopus transcription factor TFIIIA.
    Nature. 1986 Mar 20-26;320(6059):283-7 PMID: 3515197
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.19.7991
Published
1987-10-12
Pages
7991-8001
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306322
Subset
IM
Databases
GENBANK
X06252
External Links
PubMed source DOI: 10.1093/nar/15.19.7991 PMC Full Text View journal details
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