Home LiteratureArticle Details
PMID: 2836080 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The complex Arom locus of Aspergillus nidulans. Evidence for multiple gene fusions and convergent evolution.

Current genetics ·Vol. 11 ·No. 6-7 ·1987-00-00 ·Pages 491-8

Hawkins AR

Abstract

The physical positions of the DNA sequences encoding the five consecutive enzyme activities required to metabolise 3-deoxy-D-arabino-heptulosonic acid-7-phosphate to 5-enolpyruvyl-shikimate-3phosphate, which are encoded by the A. nidulans Arom polypeptide have been determined. Subfragments of the Arom locus encoding EPSP synthase and 3-dehydroquinase have been expressed in appropriate E. coli aro mutants. The DNA sequence of the A. nidulans Arom locus has been shown to have homology with the corresponding unlinked E. coli aro loci strongly suggesting (I) divergent evolution from common ancestral sequences and (II) that the complex A. nidulans Arom locus arose by multiple gene fusion. The DNA and protein sequence of the two 3-dehydroquinase isoenzymes of A. nidulans share no homology, strongly indicating separate phylogenetic origins and their convergent evolution. The 5' and 3' non-translated DNA sequence of the A. nidulans Arom locus is presented along with the presumed sites for transcription initiation and polyadenylation determined by S1 nuclease protection experiments.

MeSH Terms
3-Phosphoshikimate 1-Carboxyvinyltransferase Alkyl and Aryl Transferases Amino Acid Sequence Aspergillus nidulans/enzymology,genetics Base Sequence Genes Genes, Fungal Lyases/genetics Molecular Sequence Data Phosphorus-Oxygen Lyases Phosphotransferases/genetics Phosphotransferases (Alcohol Group Acceptor) Transferases/genetics
Chemicals
Transferases Alkyl and Aryl Transferases 3-Phosphoshikimate 1-Carboxyvinyltransferase Phosphotransferases Phosphotransferases (Alcohol Group Acceptor) shikimate kinase Lyases 3-dehydroquinate synthetase Phosphorus-Oxygen Lyases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Hawkins A R
Department of Biochemistry, University of Leicester, UK.
References (34)
34 references, click to expand
  1. Specific in vitro transcription of conalbumin gene is drastically decreased by single-point mutation in T-A-T-A box homology sequence.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7024-8 PMID: 6938951
  2. Molecular cloning of the 3-phosphoglycerate kinase (PGK) gene from Aspergillus nidulans.
    Curr Genet. 1985;9(4):293-8 PMID: 3916724
  3. Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA.
    Nature. 1983 Oct 13-19;305(5935):600-5 PMID: 6194440
  4. Cloning and characterization of the three enzyme structural genes QUTB, QUTC and QUTE from the quinic acid utilization gene cluster in Aspergillus nidulans.
    Curr Genet. 1985;9(4):305-11 PMID: 3916726
  5. Sequence analysis and transformation by the catabolic 3-dehydroquinase (QUTE) gene from Aspergillus nidulans.
    Biochem J. 1986 Dec 1;240(2):481-8 PMID: 2949740
  6. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
    EMBO J. 1982;1(8):945-51 PMID: 6329717
  7. Chemical synthesis of a gene for somatomedin C.
    Nucleic Acids Res. 1985 Apr 25;13(8):2959-77 PMID: 2987870
  8. Cloning of the ARO cluster gene of Neurospora crassa and its expression in Escherichia coli.
    Mol Gen Genet. 1985;199(3):446-51 PMID: 2993794
  9. An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels.
    Anal Biochem. 1983 Jul 1;132(1):14-9 PMID: 6312834
  10. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  11. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  12. FAD-binding site of glutathione reductase.
    J Mol Biol. 1982 Sep 15;160(2):287-308 PMID: 7175934
  13. Substrate positions and induced-fit in crystalline adenylate kinase.
    J Mol Biol. 1977 Jul;114(1):37-45 PMID: 198550
  14. The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3' termini.
    Nucleic Acids Res. 1985 Feb 25;13(4):1347-68 PMID: 2987822
  15. The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase.
    Biochem J. 1986 Sep 1;238(2):475-83 PMID: 3541912
  16. Simultaneous rapid chemical synthesis of over one hundred oligonucleotides on a microscale.
    EMBO J. 1984 Apr;3(4):801-5 PMID: 16453516
  17. Partial enzyme aggregates formed by pleiotropic mutants in the arom gene cluster of Neurospora crassa.
    Proc Natl Acad Sci U S A. 1971 Jan;68(1):58-62 PMID: 5276302
  18. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  19. Nucleotide sequence encoding the biosynthetic dehydroquinase function of the penta-functional arom locus of Aspergillus nidulans.
    Nucleic Acids Res. 1985 Nov 25;13(22):8119-28 PMID: 3906567
  20. Isolation of the ARO1 cluster gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1983 Sep;3(9):1609-14 PMID: 6355828
  21. Chemical and biological evolution of nucleotide-binding protein.
    Nature. 1974 Jul 19;250(463):194-9 PMID: 4368490
  22. The isolation and nucleotide sequence of the complex AROM locus of Aspergillus nidulans.
    Nucleic Acids Res. 1986 Mar 11;14(5):2201-13 PMID: 3515316
  23. The complete amino acid sequence of 3-dehydroquinate synthase of Escherichia coli K12.
    FEBS Lett. 1986 May 5;200(1):11-7 PMID: 3009224
  24. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  25. Nucleotide sequence of the transcription unit containing the aroL and aroM genes from Escherichia coli K-12.
    J Bacteriol. 1986 Jan;165(1):233-9 PMID: 3001025
  26. Empirical predictions of protein conformation.
    Annu Rev Biochem. 1978;47:251-76 PMID: 354496
  27. Genetical and biochemical aspects of quinate breakdown in the filamentous fungus Aspergillus nidulans.
    Biochem Genet. 1982 Apr;20(3-4):271-86 PMID: 7049157
  28. Cloning and expression in Escherichia coli K-12 of the biosynthetic dehydroquinase function of the arom cluster gene from the eucaryote, Aspergillus nidulans.
    Mol Gen Genet. 1982;186(1):145-52 PMID: 6287167
  29. Evidence for two control genes regulating expression of the quinic acid utilization (qut) gene cluster in Aspergillus nidulans.
    J Gen Microbiol. 1984 Mar;130(3):567-74 PMID: 6374025
  30. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.
    J Bacteriol. 1969 Jan;97(1):244-9 PMID: 4884815
  31. Structural and functional similarities within the coenzyme binding domains of dehydrogenases.
    J Mol Biol. 1974 Oct 25;89(2):339-54 PMID: 4374553
  32. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  33. Primary and tertiary structure studies of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Isolation and alignment of the CNBr peptides; interactions of the protein with flavin adenine dinucleotide.
    Eur J Biochem. 1980 Dec;113(1):141-50 PMID: 6780352
  34. Analysis of the structure and transcription of the aro3 cluster gene in Schizosaccharomyces pombe.
    Mol Gen Genet. 1984;195(1-2):164-9 PMID: 6092844
Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00384611
Published
1987-00-00
Pages
491-8
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Databases
GENBANK
X03180, X03684, X05204
External Links
PubMed source DOI: 10.1007/BF00384611 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2