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PMID: 3001025 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the transcription unit containing the aroL and aroM genes from Escherichia coli K-12.

Journal of bacteriology ·Vol. 165 ·No. 1 ·1986-01-00 ·Pages 233-9

DeFeyter RC, Davidson BE, Pittard J

Abstract

The nucleotide sequence of 2,021 base pairs (bp) of DNA containing the Escherichia coli aroLM operon was determined, and the coding regions of both aroL and aroM were identified. The 501-bp intercistronic region between aroL and aroM contains an open reading frame which might encode a 63-residue protein. Northern blots with RNA from strains carrying multicopy aroL+ plasmids detected one longer (2,000-base) and two shorter (950- and 1,100-base) transcripts which contained aroL. It was concluded that the longest transcript, which was not abundant, spanned the entire operon and that the shorter transcripts resulted from either termination or posttranscriptional processing in the intercistronic region. The DNA upstream of aroL contains a number of imperfect palindromes which are closely homologous to known sites of regulation by the TyrR protein in other operons.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Operon Phosphotransferases/genetics Phosphotransferases (Alcohol Group Acceptor) Transcription, Genetic Tyrosine/genetics
Chemicals
Bacterial Proteins Tyrosine Phosphotransferases Phosphotransferases (Alcohol Group Acceptor) shikimate kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
DeFeyter R C
Davidson B E
Pittard J
References (34)
34 references, click to expand
  1. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  2. Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3331-5 PMID: 6158044
  3. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  4. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  5. Metabolism of messenger RNA from the gal operon of Escherichia coli.
    J Mol Biol. 1974 Dec 15;90(3):581-99 PMID: 4615171
  6. Mitomycin C-induced expression of trpA of Salmonella typhimurium inserted into the plasmid ColE1.
    J Bacteriol. 1977 Jan;129(1):388-94 PMID: 318646
  7. A rho-dependent termination site in the gene coding for tyrosine tRNA su3 of Escherichia coli.
    Nature. 1978 Mar 30;272(5652):423-8 PMID: 345126
  8. Further procedures for sequence analysis by computer.
    Nucleic Acids Res. 1978 Mar;5(3):1013-6 PMID: 643613
  9. Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12.
    J Bacteriol. 1979 Jun;138(3):933-43 PMID: 222728
  10. Models for decay of Escherichia coli lac messenger RNA and evidence for inactivating cleavages between its messages.
    J Mol Biol. 1979 Dec 5;135(2):369-90 PMID: 94103
  11. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  12. The analysis of nucleic acids in gels using glyoxal and acridine orange.
    Methods Enzymol. 1980;65(1):380-91 PMID: 6154866
  13. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  14. RsaI: a new sequence-specific endonuclease activity from Rhodopseudomonas sphaeroides.
    J Bacteriol. 1980 May;142(2):380-3 PMID: 6247319
  15. Graphic methods to determine the function of nucleic acid sequences.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):521-38 PMID: 6364040
  16. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  17. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  18. Evidence for random endonucleolytic cleavages between messages in decay of Escherichia coli trp mRNA.
    J Mol Biol. 1980 Aug 5;141(2):227-33 PMID: 6160252
  19. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  20. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  21. Evidence for two functional gal promoters in intact Escherichia coli cells.
    J Biol Chem. 1981 Nov 25;256(22):11905-10 PMID: 6271763
  22. Codon preference and its use in identifying protein coding regions in long DNA sequences.
    Nucleic Acids Res. 1982 Jan 11;10(1):141-56 PMID: 7063399
  23. Use of the 'Perceptron' algorithm to distinguish translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2997-3011 PMID: 7048259
  24. The nucleotide sequence of aroG, the gene for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase (phe) in Escherichia coli K12.
    Nucleic Acids Res. 1982 Jul 10;10(13):4045-58 PMID: 6125934
  25. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
    Nucleic Acids Res. 1982 Aug 11;10(15):4731-51 PMID: 7133997
  26. The filamentous phage (Ff) as vectors for recombinant DNA--a review.
    Gene. 1982 Jul-Aug;19(1):1-10 PMID: 6292041
  27. Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):789-800 PMID: 6364042
  28. Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli.
    Nature. 1984 Sep 20-26;311(5983):232-5 PMID: 6090927
  29. Operator mutations of the Escherichia coli aroF gene.
    J Biol Chem. 1985 Mar 25;260(6):3820-5 PMID: 2857723
  30. Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12.
    J Mol Biol. 1984 Dec 25;180(4):1023-51 PMID: 6396419
  31. Genetic and molecular analysis of aroL, the gene for shikimate kinase II in Escherichia coli K-12.
    J Bacteriol. 1986 Jan;165(1):226-32 PMID: 3001024
  32. Purification and properties of shikimate kinase II from Escherichia coli K-12.
    J Bacteriol. 1986 Jan;165(1):331-3 PMID: 3001029
  33. Characterization of a rho-dependent termination site within the cro gene of bacteriophage lambda.
    J Biol Chem. 1980 Nov 25;255(22):11017-22 PMID: 6448859
  34. Cyclic AMP-dependent initiation and rho-dependent termination of colicin E1 gene transcription.
    J Biol Chem. 1983 Jun 10;258(11):7072-8 PMID: 6304076
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.165.1.233-239.1986
Published
1986-01-00
Pages
233-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214394
Subset
IM
Databases
GENBANK
M13045
External Links
PubMed source DOI: 10.1128/jb.165.1.233-239.1986 PMC Full Text View journal details
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