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PMID: 6247319 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

RsaI: a new sequence-specific endonuclease activity from Rhodopseudomonas sphaeroides.

Journal of bacteriology ·Vol. 142 ·No. 2 ·1980-05-00 ·Pages 380-3

Lynn SP, Cohen LK, Kaplan S, Gardner JF

Abstract

A new type II sequence-specific endonuclease, RsaI, has been identified from Rhodopseudomonas sphaeroides strain 28/5. An RsaI purification scheme that yields enzyme which is free of contaminating exonuclease and phosphatase activities after a single column fractionation has been developed. The enzyme recognized the tetranucleotide sequence 5'-GTAC-3' and cleaved between the T and A, thereby generating flush ends. RsaI should be extremely useful in deoxyribonucleic acid sequencing experiments.

MeSH Terms
Base Sequence Coliphages DNA, Bacterial/metabolism DNA, Viral/metabolism Endonucleases/isolation & purification,metabolism Oligodeoxyribonucleotides/metabolism Plasmids Rhodobacter sphaeroides/enzymology Simian virus 40
Chemicals
DNA, Bacterial DNA, Viral Oligodeoxyribonucleotides Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lynn S P
Cohen L K
Kaplan S
Gardner J F
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22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.142.2.380-383.1980
Published
1980-05-00
Pages
380-3
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC293981
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.142.2.380-383.1980 PMC Full Text View journal details
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