[Serologic AIDS diagnosis with polypeptides obtained by genetic technics of the human immunodeficiency virus (HIV-1)].

Serologische AIDS-Diagnostik mit gentechnisch gewonnenen Polypeptiden des menschlichen Immundefizienz-Virus (HIV-1).

Klinische Wochenschrift ·Vol. 65 ·No. 21 ·1987-11-02 ·Pages 1042-7

Lenz A, von Hintzenstern J, Erlwein O, Ellinger S, Bröker M, Fleckenstein B, Jahn G

Abstract

Serologic testing for human immunodeficiency virus type 1 (HIV-1) is currently based on enzyme linked immunosorbent assay (ELISA) as screening method. Positive ELISA-results have to be confirmed by at least one second procedure such as Western blotting or immunofluorescence. To obtain new diagnostic reagents for confirmatory testing, we expressed viral antigens in procaryotic systems. Peptides representing epitopes of structural core (gag)- and envelope (env)-proteins of HIV were produced in E. coli as stable immunogenic beta-galactosidase fusion proteins. Recombinant proteins were taken for immunoblot-assays. The results of Western blotting with those fusion proteins were in general comparable with conventional ELISA, immunofluorescence, immunoblot with cell-culture derived virus and commercially available ELISA tests based on recombinant proteins. Immunoblots using recombinant transmembrane protein (gp41) derived polypeptide were more sensitive than the conventional procedure with purified virion proteins. Western blotting with recombinant fusionproteins provide reliable and inexpensive serodiagnostics without handling of infectious cell cultures.

MeSH Terms

Acquired Immunodeficiency Syndrome/diagnosis,immunology Antibodies, Viral/analysis Antigens, Viral/immunology Cloning, Molecular Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Gene Products, gag HIV/immunology HIV Antibodies Humans Recombinant Fusion Proteins/immunology Recombinant Proteins/immunology Retroviridae Proteins/immunology Viral Envelope Proteins/immunology

Chemicals

Antibodies, Viral Antigens, Viral Gene Products, gag HIV Antibodies Recombinant Fusion Proteins Recombinant Proteins Retroviridae Proteins Viral Envelope Proteins

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Lenz A

Institut für Klinische und Molekulare Virologie der Universität Erlangen-Nürnberg.

von Hintzenstern J

Erlwein O

Ellinger S

Bröker M

Fleckenstein B

Jahn G

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14 references, click to expand

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Article Info

Journal

Klinische Wochenschrift

Abbr.

Klin Wochenschr

ISSN

0023-2173

DOI

10.1007/BF01726323

Published

1987-11-02

Pages

1042-7

Language

ger

Region

Germany

NLM ID

2985205R

Subset

External Links

PubMed source DOI: 10.1007/BF01726323

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