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PMID: 3323829 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Transcript analysis of the plasmid R100 traJ and finP genes.

Molecular & general genetics : MGG ·Vol. 209 ·No. 3 ·1987-10-00 ·Pages 533-44

Dempsey WB

Abstract

Single-stranded RNA probes were used to study the regulation of plasmid transfer in the infectious antibiotic resistance plasmid R100. Transcription of the positive transfer control gene traJ of R100 appears to be initiated continuously. In the presence of finO, the traJ transcript is 235 bases long, and in the absence of finO it is 1050. These sizes are strain specific. finO increases four-to tenfold the amount of the transcript from the finP gene that is detectable in cells containing R100, R136, or the sex factor F. The size of the principal finP transcript from R100 as determined on Northern blots is 105 bases. A secondary transcript with a size of 180 bases was detected in small amounts in R100 extracts. The finP transcript size was also determined by nuclease protection experiments. In this case the size was 74 bases. The 5' ends of the finP and traJ transcripts were located by primer extension experiments. A new model of FinO/P control is proposed.

MeSH Terms
Base Sequence Cloning, Molecular Escherichia coli/genetics Genes, Bacterial Molecular Weight Nucleic Acid Hybridization R Factors Transcription, Genetic
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Dempsey W B
Research Service, Veterans Administration Medical Center, TX.
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38 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331160
Published
1987-10-00
Pages
533-44
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIAID NIH HHS · AI-17402 · United States
External Links
PubMed source DOI: 10.1007/BF00331160
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