Selection against expression of the Escherichia coli gene gpt in hprt+ mouse teratocarcinoma and hybrid cells.
Thioxanthine is toxic for mammalian cells transformed by the dominant selectable marker gpt. It allowed us to select, in the presence of the endogenous hypoxanthine-guanine phosphoribosyltransferase gene, mutants that did not express gpt any more and also hybrid cells that had lost the chromosome carrying it. The gpt marker is thus dominant in negative as well as in positive selection, which makes it potentially very useful for genetic studies of mammalian cells.
13 references, click to expand
-
Stable transformation of mouse teratocarcinoma stem cells with the dominant selective marker Eco.gpt and retention of their developmental potentialities.EMBO J. 1983;2(2):229-32 PMID: 11894931
-
Expression of a bacterial gene in mammalian cells.Science. 1980 Sep 19;209(4463):1422-7 PMID: 6251549
-
Patterns of chromosome segregation in Chinese hamster x mouse cell hybrids between permanent cell lines and thymus cells.J Cell Physiol. 1980 Sep;104(3):403-13 PMID: 7419611
-
Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072-6 PMID: 7017722
-
The use of a cloned bacterial gene to study mutation in mammalian cells.Mutat Res. 1983 Sep;111(1):9-23 PMID: 6621577
-
Detection of deletion mutations in pSV2gpt-transformed cells.Mol Cell Biol. 1984 Jul;4(7):1411-5 PMID: 6095070
-
Expression of the human beta-globin gene in mouse teratocarcinoma cells.Mol Gen Genet. 1987 Jun;208(1-2):357-60 PMID: 3475529
-
Integration of a dominant selectable marker into human chromosomes and transfer of marked chromosomes to mouse cells by microcell fusion.Somat Cell Mol Genet. 1985 Mar;11(2):177-87 PMID: 3885417
-
Benzo[a]pyrene diol-epoxides: different mutagenic efficiency in human and bacterial cells.Mutat Res. 1985 Oct;152(1):5-14 PMID: 3900713
-
Human proto-oncogene c-mos maps to 8q11.EMBO J. 1985 Sep;4(9):2245-8 PMID: 3000766
-
Efficient recovery and sequencing of mutant genes from mammalian chromosomal DNA.Proc Natl Acad Sci U S A. 1986 May;83(10):3356-60 PMID: 3458187
-
Evidence for reactive oxygen species inducing mutations in mammalian cells.Proc Natl Acad Sci U S A. 1986 Dec;83(24):9616-20 PMID: 2432598
-
Selective transfer of individual human chromosomes to recipient cells.Mol Cell Biol. 1985 Jan;5(1):140-6 PMID: 3982414
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
