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PMID: 333399 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Studies on gene control regions. VI. The 5- methyl of thymine, a lac repressor recognition site.

Nucleic acids research ·Vol. 4 ·No. 9 ·1977-09-00 ·Pages 3039-54

Goeddel DV, Yansura DG, Caruthers MH

Abstract

Three site specific deoxyuridine analogs of lac operator were tested for binding with wild type (SQ) and tight binding (QX86) lac repressors. Insertion of uracil for thymine at site 13 (our nomenclature) significantly reduced the dissociation half-life of QX86 repressor for lac operator DNA (21 vs 1.2 min). Two other sites (6 and 7) are affected to a much lesser extent.

MeSH Terms
Base Sequence DNA, Bacterial Escherichia coli/drug effects,genetics Genes Kinetics Lactose Factors Oligodeoxyribonucleotides/analysis Plasmids Thymine/analogs & derivatives,pharmacology
Chemicals
DNA, Bacterial Oligodeoxyribonucleotides Thymine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Goeddel D V
Yansura D G
Caruthers M H
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.9.3039
Published
1977-09-00
Pages
3039-54
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342633
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.9.3039 PMC Full Text View journal details
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