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PMID: 3335397 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Sequence of the mouse Q4 class I gene and characterization of the gene product.

Immunogenetics ·Vol. 27 ·No. 2 ·1988-00-00 ·Pages 79-86

Robinson PJ, Bevec D, Mellor AL, Weiss EH

Abstract

The Q4 class I gene has been shown to participate in gene conversion events within the mouse major histocompatibility complex. Its complete genomic nucleotide sequence has been determined. The 5' half of Q4 resembles H-2 genes more strongly than other Q genes. Its 3' end, in contrast, is Q-like and contains a translational stop signal in exon 5 which predicts a polypeptide with an incomplete membrane spanning segment. The presence of two inverted B1 repeats suggests that part of the Q4 gene may be mobile within the genome. Gene transfer experiments have shown that the Q4 gene encodes a beta 2-microglobulin associated polypeptide of Mr 41,000. A similar protein was found in activated mouse spleen cells. The Q4 polypeptide was found to be secreted both by spleen cells and by transfected fibroblasts and was not detectable on the cell surface. Antibody binding and two-dimensional gel electrophoresis indicate that the Q4 molecule is identical to a mouse class I polypeptide, Qb-1, which has been previously described.

MeSH Terms
Amino Acid Sequence Animals Antigens, Surface/genetics Base Sequence Cloning, Molecular Cosmids Genes Genes, MHC Class I Glycoproteins/genetics Histocompatibility Antigens Class I Mice Molecular Sequence Data Promoter Regions, Genetic Pseudogenes Sequence Homology, Nucleic Acid
Chemicals
Antigens, Surface Glycoproteins Histocompatibility Antigens Class I Q surface antigens
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Robinson P J
Department of Immunology, German Cancer Research Center, Heidelberg.
Bevec D
Mellor A L
Weiss E H
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00351079
Published
1988-00-00
Pages
79-86
Language
English
Region
United States
NLM ID
0420404
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00351079 View journal details
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