Home LiteratureArticle Details
PMID: 3353370 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The alpha and beta chains of human platelet glycoprotein Ib are both transmembrane proteins containing a leucine-rich amino acid sequence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 7 ·1988-04-00 ·Pages 2135-9

Lopez JA, Chung DW, Fujikawa K, Hagen FS, Davie EW, Roth GJ

Abstract

The primary structure of the beta chain of human glycoprotein Ib (GPIb), the platelet receptor for von Willebrand factor, has been established by a combination of cDNA cloning and amino acid sequence analysis. A lambda phage cDNA expression library prepared from human erythroleukemia cells (HEL cells) was screened with a radiolabeled affinity-purified rabbit polyclonal antibody to the beta chain of GPIb. Eighteen positive clones were isolated and plaque-purified and the nucleotide sequences of three were determined. The composite sequence spanned 968 nucleotides and included a 5' untranslated region of 22 nucleotides, an open reading frame of 618 nucleotides encoding a signal peptide of 28 amino acids and a mature protein of 181 amino acids, a stop codon, and a 3' noncoding region of 307 nucleotides. The 3' noncoding sequence also contained a polyadenylylation signal (AATAAA) 14 nucleotides upstream from the poly(A) tail of 18 nucleotides. Edman degradation of the intact beta chain and of peptides produced by chemical cleavage yielded amino acid sequences spanning 76 residues that were identical to those predicted from the cDNA. The amino-terminal region of the beta chain contains a leucine-rich sequence of 24 amino acids that is similar to a sequence that occurs as seven tandem repeats in the alpha chain of GPIb and nine tandem repeats in leucine-rich alpha 2-glycoprotein. The leucine-rich sequence in the beta chain of GPIb is flanked on both sides by amino acid sequences that are similar to those flanking the leucine-rich tandem repeats of the alpha chain of GPIb and leucine-rich alpha 2-glycoprotein. The amino-terminal region of the beta chain of GPIb is followed by a transmembrane segment of 25 amino acids and an intracellular segment of 34 amino acids at the carboxyl terminus of the protein. The intracellular segment contains an unpaired cysteine and two potential sites for phosphorylation by cAMP-dependent protein kinase.

MeSH Terms
Amino Acid Sequence Base Sequence DNA/genetics Humans Leucine/analysis Molecular Sequence Data Platelet Membrane Glycoproteins/genetics Sequence Homology, Nucleic Acid
Chemicals
Platelet Membrane Glycoproteins DNA Leucine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lopez J A
Department of Biochemistry, University of Washington, Seattle.
Chung D W
Fujikawa K
Hagen F S
Davie E W
Roth G J
References (37)
37 references, click to expand
  1. Modification of the single tryptophan residue of staphylococcal nuclease by a new mild oxidizing agent.
    J Biol Chem. 1970 Apr 25;245(8):1895-902 PMID: 4985905
  2. Analysis of a major human chorionic somatomammotropin gene. Evidence for two functional promoter elements.
    J Biol Chem. 1984 Nov 10;259(21):13131-8 PMID: 6208192
  3. Platelet plasma membrane glycoproteins. Evidence for the presence of nonequivalent disulfide bonds using nonreduced-reduced two-dimensional gel electrophoresis.
    J Biol Chem. 1977 Mar 25;252(6):2121-6 PMID: 845165
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Platelet glycocalicin. Interaction with thrombin and role as thrombin receptor of the platelet surface.
    J Biol Chem. 1978 May 25;253(10):3435-43 PMID: 418063
  6. Isolation of human platelet glycoproteins.
    Biochim Biophys Acta. 1979 Aug 23;555(3):442-59 PMID: 486461
  7. The von Willebrand factor-binding domain of platelet membrane glycoprotein Ib. Characterization by monoclonal antibodies and partial amino acid sequence analysis of proteolytic fragments.
    J Biol Chem. 1986 Sep 25;261(27):12579-85 PMID: 2943738
  8. On the association of glycoprotein Ib and actin-binding protein in human platelets.
    J Cell Biol. 1985 Jan;100(1):317-21 PMID: 3155520
  9. Periodicity of leucine and tandem repetition of a 24-amino acid segment in the primary structure of leucine-rich alpha 2-glycoprotein of human serum.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):1906-10 PMID: 3856868
  10. Purification and preliminary characterization of the glycoprotein Ib complex in the human platelet membrane.
    Eur J Biochem. 1985 Sep 16;151(3):637-49 PMID: 3161731
  11. Signal sequences. The limits of variation.
    J Mol Biol. 1985 Jul 5;184(1):99-105 PMID: 4032478
  12. Structure and function of platelet membrane glycoproteins Ib and V. Effects of leukocyte elastase and other proteases on platelets response to von Willebrand factor and thrombin.
    Eur J Biochem. 1985 Nov 15;153(1):1-11 PMID: 2933256
  13. Thiol-specific probes indicate that the beta-chain of platelet glycoprotein Ib is a transmembrane protein with a reactive endofacial sulfhydryl group.
    Biochemistry. 1985 Sep 24;24(20):5430-6 PMID: 2934089
  14. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  15. Glycoprotein Ib beta is the only phosphorylated major membrane glycoprotein in human platelets.
    Biochem J. 1986 Mar 1;234(2):373-9 PMID: 2941010
  16. Expression of platelet glycoprotein Ib alpha in HEL cells.
    J Biol Chem. 1986 Dec 5;261(34):15854-62 PMID: 3465723
  17. Determinants of messenger RNA stability.
    Cell. 1987 Jan 16;48(1):5-6 PMID: 2431794
  18. The human growth hormone gene locus: structure, evolution, and allelic variations.
    DNA. 1987 Feb;6(1):59-70 PMID: 3030680
  19. Biosynthesis of major platelet proteins in human blood platelets.
    Eur J Biochem. 1987 Apr 1;164(1):189-95 PMID: 3830180
  20. Amino acid sequence of the von Willebrand factor-binding domain of platelet membrane glycoprotein Ib.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5610-4 PMID: 3497398
  21. Cloning of the alpha chain of human platelet glycoprotein Ib: a transmembrane protein with homology to leucine-rich alpha 2-glycoprotein.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5615-9 PMID: 3303030
  22. Protein serine/threonine kinases.
    Annu Rev Biochem. 1987;56:567-613 PMID: 2956925
  23. Identification of glycoprotein Ib beta as one of the major proteins phosphorylated during exposure of intact platelets to agents that activate cyclic AMP-dependent protein kinase.
    J Biol Chem. 1987 Sep 15;262(26):12627-31 PMID: 3040761
  24. Isolation and characterization of the alpha and beta chains of human platelet glycoprotein Ib.
    Biochem Biophys Res Commun. 1987 Sep 15;147(2):526-34 PMID: 3632685
  25. Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen).
    Anal Biochem. 1981 Oct;117(1):136-46 PMID: 7316186
  26. Evidence that a human beta-tubulin pseudogene is derived from its corresponding mRNA.
    Nature. 1982 May 6;297(5861):83-4 PMID: 7070533
  27. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  28. Evidence of normal functional levels of activated protein C inhibitor in combined Factor V/VIII deficiency disease.
    J Clin Invest. 1982 Dec;70(6):1260-72 PMID: 6294139
  29. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  30. Conversion of RNA to DNA in mammals: Alu-like elements and pseudogenes.
    Nature. 1983 Feb 10;301(5900):471-2 PMID: 6185852
  31. Efficient isolation of genes by using antibody probes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
  32. Patterns of amino acids near signal-sequence cleavage sites.
    Eur J Biochem. 1983 Jun 1;133(1):17-21 PMID: 6852022
  33. Calcium-dependent proteolysis occurs during platelet aggregation.
    J Biol Chem. 1983 Aug 25;258(16):9973-81 PMID: 6309792
  34. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  35. Recycling of platelet phosphorylation and cytoskeletal assembly.
    J Cell Biol. 1984 Jan;98(1):8-15 PMID: 6423649
  36. Cysteines in the transmembrane region of major histocompatibility complex antigens are fatty acylated via thioester bonds.
    J Biol Chem. 1984 Jun 10;259(11):7230-8 PMID: 6373770
  37. The chromatographic determination of cystine and cysteine residues in proteins as s-beta-(4-pyridylethyl)cysteine.
    J Biol Chem. 1970 Aug 10;245(15):3868-71 PMID: 5492953
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.7.2135
Published
1988-04-00
Pages
2135-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279943
Subset
IM
Grants
NHLBI NIH HHS · HL 16919 · United States
Databases
GENBANK
J03259
External Links
PubMed source DOI: 10.1073/pnas.85.7.2135 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2