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PMID: 3362864 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Qa-region class I gene expression: identification of a second class I gene, Q9, encoding a Qa-2 polypeptide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 9 ·1988-05-00 ·Pages 3100-4

Soloski MJ, Hood L, Stroynowski I

Abstract

A feature of the expression of the tissue-specific class I antigen Qa-2 is the quantitative variation among mouse strains. Recently, the class I gene Q7 has been shown to encode a protein product that is biochemically indistinguishable from the lymphocyte-bound Qa-2 molecule. Utilizing gene transfection, we have identified a second Qa-2 subregion class I gene (Q9), in H-2b mice, which encodes a polypeptide biochemically similar to the Q7 and the Qa-2 polypeptides. Furthermore, we have observed that cell lines transfected with the allelic forms of the Q7 gene from C57BL/10 (Qa-2hi) or BALB/C (Qa-2low) display quantitative differences in cell-surface expression. Based on these studies, we suggest that gene dosage and allele-specific variation in cell-surface expression contribute to the strain-specific variation in the levels of Qa-2 antigen expression.

MeSH Terms
Alleles Animals Antigens, Surface/genetics Gene Expression Regulation Genes, MHC Class I H-2 Antigens/analysis Histocompatibility Antigens/genetics Histocompatibility Antigens Class I Lipase/metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL Protein Processing, Post-Translational Transcription, Genetic Transfection Type C Phospholipases/metabolism
Chemicals
Antigens, Surface H-2 Antigens H-2Kb protein, mouse Histocompatibility Antigens Histocompatibility Antigens Class I Q surface antigens Lipase Type C Phospholipases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Soloski M J
Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Hood L
Stroynowski I
References (31)
31 references, click to expand
  1. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 PMID: 286319
  2. A single gene encodes soluble and membrane-bound forms of the major histocompatibility Qa-2 antigen: anchoring of the product by a phospholipid tail.
    Cell. 1987 Aug 28;50(5):759-68 PMID: 2441874
  3. A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains.
    Cell. 1981 Sep;25(3):683-92 PMID: 6895187
  4. Qualitative and quantitative aspects of anti-H-2Ld sera.
    Transplantation. 1981 Aug;32(2):128-36 PMID: 7292590
  5. Further biochemical data on Qa-2.
    Immunogenetics. 1981;14(1-2):129-40 PMID: 6173317
  6. Clusters of genes encoding mouse transplantation antigens.
    Cell. 1982 Mar;28(3):489-98 PMID: 6280871
  7. Description of a Qa-2 like alloantigen (Qa-m2).
    Eur J Immunol. 1982 May;12(5):374-9 PMID: 6980125
  8. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  9. Monoclonal antibody reveals H-2-linked quantitative and qualitative variation in the expression of a Qa-2 region determinant.
    Immunogenetics. 1983;17(3):303-16 PMID: 6601054
  10. Genetic mapping in the major histocompatibility complex by restriction enzyme site polymorphisms: most mouse class I genes map to the Tla complex.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3425-9 PMID: 6304710
  11. Serologic cross-reactivity between Class I MHC molecules and an H-2-linked differentiation antigen as detected by monoclonal antibodies.
    J Exp Med. 1984 Jan 1;159(1):21-40 PMID: 6363595
  12. Organization and evolution of the class I gene family in the major histocompatibility complex of the C57BL/10 mouse.
    Nature. 1984 Aug 23-29;310(5979):650-5 PMID: 6088985
  13. Molecular weight diversity among murine class I antigens: both the mature cell surface forms and the unglycosylated polypeptides vary significantly in molecular weight.
    Mol Immunol. 1985 Feb;22(2):155-8 PMID: 3974579
  14. Expression and T cell recognition of hybrid antigens with amino-terminal domains encoded by Qa-2 region of major histocompatibility complex and carboxyl termini of transplantation antigens.
    J Exp Med. 1985 May 1;161(5):935-52 PMID: 2580938
  15. Secretion of a soluble class I molecule encoded by the Q10 gene of the C57BL/10 mouse.
    EMBO J. 1985 Feb;4(2):369-74 PMID: 4018029
  16. Qa antigens and their differential distribution on lymphoid, myeloid and stem cells.
    J Immunogenet. 1984 Oct-Dec;11(5-6):265-81 PMID: 6399516
  17. Structure and expression of genes encoding murine Qa-2 class I antigens.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5920-4 PMID: 3862106
  18. Qb-1, a new class I polypeptide encoded by the Qa region of the mouse H-2 complex.
    Immunogenetics. 1985;22(3):285-9 PMID: 4044021
  19. Phosphatidylinositol is the membrane-anchoring domain of the Thy-1 glycoprotein.
    Nature. 1985 Nov 7-13;318(6041):62-4 PMID: 2865681
  20. Class I genes and molecules: an update.
    Immunology. 1986 Jan;57(1):3-18 PMID: 2417940
  21. Duplicated gene pairs and alleles of class I genes in the Qa2 region of the murine major histocompatibility complex: a comparison.
    EMBO J. 1985 Dec 1;4(12):3203-7 PMID: 3004940
  22. Qa gene expression: biosynthesis and secretion of Qa-2 molecules in activated T cells.
    Proc Natl Acad Sci U S A. 1986 May;83(9):2949-53 PMID: 3486423
  23. Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes.
    Nature. 1986 Jul 10-16;322(6075):181-4 PMID: 2873512
  24. Biochemical genetics of TL antigens.
    Immunogenetics. 1986;24(2):103-14 PMID: 2427440
  25. Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies.
    J Exp Med. 1986 Sep 1;164(3):709-22 PMID: 3489060
  26. Structural characterization of the TAP molecule: a phosphatidylinositol-linked glycoprotein distinct from the T cell receptor/T3 complex and Thy-1.
    Cell. 1986 Nov 7;47(3):365-70 PMID: 2876780
  27. Two different biosynthetic pathways for the secretion of Qa region-associated class I antigens by mouse lymphocytes.
    Proc Natl Acad Sci U S A. 1987 Jan;84(2):527-31 PMID: 3491993
  28. Tissue-specific expression of cell-surface Qa-2 antigen from a transfected Q7b gene of C57BL/10 mice.
    J Exp Med. 1987 May 1;165(5):1358-70 PMID: 3502706
  29. Regulation of murine class I genes by interferons is controlled by regions located both 5' and 3' to the transcription initiation site.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3380-4 PMID: 3106967
  30. Removal of lymphocyte surface molecules with phosphatidylinositol-specific phospholipase C: effects on mitogen responses and evidence that ThB and certain Qa antigens are membrane-anchored via phosphatidylinositol.
    J Immunol. 1987 Jun 1;138(11):3877-84 PMID: 2953793
  31. Phosphatidylinositol-specific phospholipase C from Staphylococcus aureus.
    Methods Enzymol. 1981;71 Pt C:741-6 PMID: 7278681
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.9.3100
Published
1988-05-00
Pages
3100-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280151
Subset
IM
Grants
NIAID NIH HHS · AI 19624 · United States
NIAID NIH HHS · AI 20922 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.9.3100 PMC Full Text View journal details
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