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PMID: 337105 Published · ppublish English Journal Article

Fine structure physical mapping of 4S RNA genes on mitochondrial DNA of Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 154 ·No. 3 ·1977-09-09 ·Pages 255-62

Van Ommen GJ, Groot GS, Borst P

Abstract

We have localized the genes for mitochondrial 4S RNA on the physical map of the mtDNA of several Saccharomyces cerevisiae strains by hybridization of iodinated 4S RNA to the restriction fragments obtained with endonucleases HindII + III, EcoRI and HapII. The data indicate that 5-8 of the 4S RNA genes are dispersed over a large area of the genome whereas the rest (about 18 genes) is located within an area of about 9000 bp in length (about 18 genes) is located within an area of about 9000 bp in length (about 12% of the genome) between the markers for chloramphenicol and paromomycin resistance (RIB 1 and PAR 1 loci). Within this region a cluster is present of 5 genes on a DNA fragment of 460 bp.

MeSH Terms
DNA/metabolism DNA, Mitochondrial Endonucleases/metabolism Extrachromosomal Inheritance Genes Genetic Linkage Mutation Nucleic Acid Hybridization RNA, Ribosomal Saccharomyces cerevisiae/enzymology,genetics
Chemicals
DNA, Mitochondrial RNA, Ribosomal DNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Van Ommen G J
Groot G S
Borst P
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21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00571280
Published
1977-09-09
Pages
255-62
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00571280
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