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PMID: 3380691 Published · ppublish English Journal Article

The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones.

Nucleic acids research ·Vol. 16 ·No. 10 ·1988-05-25 ·Pages 4637-50

Jacobs KA, Rudersdorf R, Neill SD, Dougherty JP, Brown EL, Fritsch EF

Abstract

In solutions of tetraalkylammonium salts the melting temperature of oligonucleotide duplexes is independent of nucleotide sequence and thus GC content. Data quantitating the destabilizing effects of various mismatches in these solvents are also presented. The results are in accord with theories on DNA melting and establish conditions under which oligonucleotides can be used as hybridization probes with predictable and controllable specificity.

MeSH Terms
Base Sequence DNA, Recombinant Drug Stability Indicators and Reagents Nucleic Acid Denaturation Oligodeoxyribonucleotides/chemical synthesis Quaternary Ammonium Compounds Thermodynamics
Chemicals
DNA, Recombinant Indicators and Reagents Oligodeoxyribonucleotides Quaternary Ammonium Compounds
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jacobs K A
Genetics Institute Inc., Cambridge, MA 02140.
Rudersdorf R
Neill S D
Dougherty J P
Brown E L
Fritsch E F
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.10.4637
Published
1988-05-25
Pages
4637-50
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336654
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.10.4637 PMC Full Text View journal details
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