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PMID: 3384800 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cloning and nucleotide sequence of the type E staphylococcal enterotoxin gene.

Journal of bacteriology ·Vol. 170 ·No. 7 ·1988-07-00 ·Pages 2954-60

Couch JL, Soltis MT, Betley MJ

Abstract

The gene for staphylococcal enterotoxin type E (entE) was cloned from Staphylococcus aureus into plasmid vector pBR322 and introduced into Escherichia coli. A staphylococcal enterotoxin type E-producing E. coli strain was isolated. The complete nucleotide sequence of the cloned structural entE gene and the N-terminal amino acid sequence of mature staphylococcal enterotoxin type E were determined. The entE gene contained 771 base pairs that encoded a protein with a molecular weight of 29,358 which was apparently processed to a mature extracellular form with a molecular weight of 26,425. DNA sequence comparisons indicated that staphylococcal enterotoxins type E and A are closely related. There was 84% nucleotide sequence homology between entE and the gene for staphylococcal enterotoxin type A; these genes encoded protein products that had 214 (83%) homologous amino acid residues (mature forms had 188 [82%] homologous amino acid residues).

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Bacterial/genetics Enterotoxins/genetics Genes Genes, Bacterial Molecular Sequence Data Nucleic Acid Hybridization Sequence Homology, Nucleic Acid Staphylococcus aureus/genetics
Chemicals
DNA, Bacterial Enterotoxins enterotoxin E, Staphylococcal enterotoxin A, Staphylococcal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Couch J L
Department of Bacteriology, University of Wisconsin-Madison 53706.
Soltis M T
Betley M J
References (39)
39 references, click to expand
  1. The primary structure of staphylococcal enterotoxin B. 3. The cyanogen bromide peptides of reduced and aminoethylated enterotoxin B, and the complete amino acid sequence.
    J Biol Chem. 1970 Jul 25;245(14):3518-25 PMID: 5470821
  2. Nucleotide sequence of the type A staphylococcal enterotoxin gene.
    J Bacteriol. 1988 Jan;170(1):34-41 PMID: 3335483
  3. Studies on plasmid replication. I. Plasmid incompatibility and establishment in Staphylococcus aureus.
    J Mol Biol. 1972 Jul 21;68(2):285-302 PMID: 4262657
  4. Restriction of bacteriophage lambda by Escherichia coli K.
    J Mol Biol. 1973 Dec 15;81(3):395-407 PMID: 4767461
  5. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  6. Detecting the enterotoxigenicity of Staphylococcus aureus strains.
    Appl Microbiol. 1974 Dec;28(6):946-50 PMID: 4217590
  7. A freeze-squeeze method for recovering long DNA from agarose gels.
    Anal Biochem. 1975 May 26;66(1):213-20 PMID: 1096670
  8. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  9. Two restriction and modification systems in Staphylococcus aureus NCTC8325.
    J Gen Microbiol. 1976 Oct;96(2):277-81 PMID: 136497
  10. Occurrence of a class II restriction endonuclease in Staphylococcus aureus.
    J Bacteriol. 1977 Aug;131(2):645-9 PMID: 885840
  11. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  12. Isolation of specific and common antibodies to staphylococcal enterotoxins A and E by affinity chromatography.
    Infect Immun. 1978 Aug;21(2):387-91 PMID: 689729
  13. Isolation of specific and common antibodies to staphylococcal enterotoxins B, C1, and C2.
    Infect Immun. 1980 Feb;27(2):431-4 PMID: 7380538
  14. Streptococcal toxins (streptolysin O, streptolysin S, erythrogenic toxin).
    Pharmacol Ther. 1980;11(3):661-717 PMID: 7003609
  15. A gas-liquid solid phase peptide and protein sequenator.
    J Biol Chem. 1981 Aug 10;256(15):7990-7 PMID: 7263636
  16. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  17. Mechanism of incorporation of cell envelope proteins in Escherichia coli.
    Annu Rev Microbiol. 1982;36:435-65 PMID: 6756294
  18. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  19. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  20. Confirmation of protoplast fusion-derived linkages in Staphylococcus aureus by transformation with protoplast DNA.
    J Bacteriol. 1983 Apr;154(1):406-12 PMID: 6572625
  21. The complete amino acid sequence of staphylococcal enterotoxin C1.
    J Biol Chem. 1983 May 25;258(10):6300-6 PMID: 6189824
  22. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  23. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
    Gene. 1983 Dec;26(1):101-6 PMID: 6323249
  24. Group A streptococcal phage T12 carries the structural gene for pyrogenic exotoxin type A.
    Mol Gen Genet. 1984;194(1-2):52-6 PMID: 6374381
  25. Identification, purification, and some physicochemical properties of staphylococcal enterotoxin C3.
    Infect Immun. 1984 Sep;45(3):625-30 PMID: 6469349
  26. Staphylococcal enterotoxin A gene is associated with a variable genetic element.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5179-83 PMID: 6089183
  27. The gene for type A streptococcal exotoxin (erythrogenic toxin) is located in bacteriophage T12.
    Infect Immun. 1984 Nov;46(2):531-6 PMID: 6389348
  28. Production of monoclonal antibodies to staphylococcal enterotoxin A.
    Appl Environ Microbiol. 1984 Dec;48(6):1171-5 PMID: 6517583
  29. Penicillinase plasmid-linked genetic determinants for enterotoxins B and C1 production in Staphylococcus aureus.
    Infect Immun. 1985 Feb;47(2):514-21 PMID: 3871425
  30. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  31. Staphylococcal enterotoxin A is encoded by phage.
    Science. 1985 Jul 12;229(4709):185-7 PMID: 3160112
  32. TnphoA: a transposon probe for protein export signals.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):8129-33 PMID: 2999794
  33. Nucleotide sequence of the type A streptococcal exotoxin (erythrogenic toxin) gene from Streptococcus pyogenes bacteriophage T12.
    Infect Immun. 1986 Apr;52(1):144-50 PMID: 3514452
  34. Nucleotide sequence of the enterotoxin B gene from Staphylococcus aureus.
    J Bacteriol. 1986 Apr;166(1):29-33 PMID: 3957869
  35. Detection of staphylococcal enterotoxins by enzyme-linked immunosorbent assays and radioimmunoassays: comparison of monoclonal and polyclonal antibody systems.
    Appl Environ Microbiol. 1986 May;51(5):885-90 PMID: 3729391
  36. Streptococcal pyrogenic exotoxin type A (scarlet fever toxin) is related to Staphylococcus aureus enterotoxin B.
    Mol Gen Genet. 1986 May;203(2):354-6 PMID: 3526093
  37. Complete amino acid sequence of staphylococcal enterotoxin A.
    J Biol Chem. 1987 May 25;262(15):7006-13 PMID: 3584106
  38. Nucleotide sequence of the staphylococcal enterotoxin C1 gene and relatedness to other pyrogenic toxins.
    Mol Gen Genet. 1987 Aug;209(1):15-20 PMID: 2823067
  39. Effects of the recipient strain and ultraviolet irradiation on transduction kinetics of the penicillinase plasmid of Staphylococcus aureus.
    J Bacteriol. 1971 Dec;108(3):1192-9 PMID: 4257946
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.7.2954-2960.1988
Published
1988-07-00
Pages
2954-60
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211234
Subset
IM
Grants
NIAID NIH HHS · AI23036 · United States
Databases
GENBANK
M21319
External Links
PubMed source DOI: 10.1128/jb.170.7.2954-2960.1988 PMC Full Text View journal details
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