Home LiteratureArticle Details
PMID: 3402440 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural relationships between clathrin assembly proteins from the Golgi and the plasma membrane.

The EMBO journal ·Vol. 7 ·No. 4 ·1988-04-00 ·Pages 919-29

Ahle S, Mann A, Eichelsbacher U, Ungewickell E

Abstract

We have established by peptide mapping and immunochemical analysis of purified clathrin assembly protein preparations from bovine brain, that the cluster of components of mol. wt 100-120 kd fall into four classes, which we term alpha, beta, beta' and gamma. The beta and beta' proteins are immunologically related and generate a series of common tryptic peptides. The same criteria reveal no such homologies between the alpha, beta(beta') and gamma polypeptides. The so-called HA-II assembly protein group contains equimolar amounts of alpha and beta class polypeptides, which are shown to interact with each other. In the HA-I group assembly protein complex gamma and beta' class polypeptides form a stoichiometric complex. Immunofluorescence microscopy reveals that the HA-I complex is specifically associated with clathrin-coated membranes in the Golgi region of cultured cells, whereas the HA-II complex appears to be restricted to coated pits on the plasma membrane. The data lead to the tentative conclusion that the clathrin assembly proteins are involved in the recognition of the intracellular targets by uncoated vesicles.

MeSH Terms
Animals Brain/metabolism Cattle Cell Membrane/metabolism Clathrin/biosynthesis,genetics,isolation & purification Electrophoresis, Polyacrylamide Gel Golgi Apparatus/metabolism Macromolecular Substances Molecular Weight Peptide Fragments/analysis Protein Processing, Post-Translational
Chemicals
Clathrin Macromolecular Substances Peptide Fragments
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ahle S
Max-Planck-Institut für Biochemie, Martinsried b. München, FRG.
Mann A
Eichelsbacher U
Ungewickell E
References (36)
36 references, click to expand
  1. Quantification of Coomassie Blue stained proteins in polyacrylamide gels based on analyses of eluted dye.
    Anal Biochem. 1975 Feb;63(2):595-602 PMID: 47719
  2. Purification and characterization of two distinct complexes of assembly polypeptides from calf brain coated vesicles that differ in their polypeptide composition and kinase activities.
    J Biol Chem. 1987 Sep 5;262(25):12182-8 PMID: 2887566
  3. Tropomyosin from bovine brain contains two polypeptide chains of slightly different molecular weights.
    FEBS Lett. 1978 Jan 1;85(1):145-8 PMID: 620785
  4. Coated vesicles: characterization, selective dissociation, and reassembly.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4394-8 PMID: 30086
  5. Immunocytochemical visualization of coated pits and vesicles in human fibroblasts: relation to low density lipoprotein receptor distribution.
    Cell. 1978 Nov;15(3):919-33 PMID: 215316
  6. On the structural and functional components of coated vesicles.
    J Mol Biol. 1978 Dec 25;126(4):803-12 PMID: 218020
  7. Mechanochemical properties of brain clathrin: interactions with actin and alpha-actinin and polymerization into basketlike structures or filaments.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):116-20 PMID: 34147
  8. Clathrin-coated vesicles: isolation, dissociation and factor-dependent reassociation of clathrin baskets.
    Cell. 1979 Feb;16(2):303-12 PMID: 455437
  9. Assembly units of clathrin coats.
    Nature. 1981 Jan 29;289(5796):420-2 PMID: 7464911
  10. Membrane recycling by coated vesicles.
    Annu Rev Biochem. 1981;50:85-101 PMID: 7023370
  11. Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
    J Biol Chem. 1981 Dec 10;256(23):11955-7 PMID: 7028745
  12. The binding of clathrin triskelions to membranes from coated vesicles.
    Cell. 1981 Nov;26(3 Pt 1):439-46 PMID: 7034962
  13. Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulin-containing structures.
    Methods Cell Biol. 1982;24:97-132 PMID: 7048022
  14. Isolation and preliminary characterization of clathrin-associated proteins.
    Eur J Biochem. 1982 Jul;125(2):463-70 PMID: 7117245
  15. The 'cell monolayer technique' in membrane research.
    Eur J Cell Biol. 1982 Aug;28(1):160-72 PMID: 6181998
  16. Golgi detection in mitotic and interphase cells by antibodies to secreted galactosyltransferase.
    Exp Cell Res. 1982 Nov;142(1):85-94 PMID: 6814941
  17. Monoclonal antibodies to desmin, the muscle-specific intermediate filament protein.
    EMBO J. 1983;2(12):2305-12 PMID: 6421570
  18. An enzyme that removes clathrin coats: purification of an uncoating ATPase.
    J Cell Biol. 1984 Aug;99(2):723-33 PMID: 6146630
  19. Identification of a protein kinase as an intrinsic component of rat liver coated vesicles.
    Biochemistry. 1984 Sep 11;23(19):4420-6 PMID: 6148963
  20. Purification and properties of 100-kd proteins from coated vesicles and their reconstitution with clathrin.
    EMBO J. 1984 Sep;3(9):1951-7 PMID: 6149117
  21. Clathrin-immunoreactive sites in the Golgi apparatus are concentrated at the trans pole in polypeptide hormone-secreting cells.
    Proc Natl Acad Sci U S A. 1985 Aug;82(16):5385-9 PMID: 3895230
  22. Limited proteolytic digestion of coated vesicle assembly polypeptides abolishes reassembly activity.
    J Cell Biochem. 1985;28(1):47-58 PMID: 2863276
  23. Assembly of the mannose-6-phosphate receptor into reconstituted clathrin coats.
    EMBO J. 1985 Oct;4(10):2457-60 PMID: 2865131
  24. Immunofluorescent localization of 100K coated vesicle proteins.
    J Cell Biol. 1986 Jan;102(1):48-54 PMID: 2867098
  25. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
    EMBO J. 1985 Dec 16;4(13A):3385-91 PMID: 2868889
  26. The phosphorylation of coated membrane proteins in intact neurons.
    J Cell Biol. 1986 Apr;102(4):1325-33 PMID: 2870069
  27. Clathrin-coated vesicles contain two protein kinase activities. Phosphorylation of clathrin beta-light chain by casein kinase II.
    J Biol Chem. 1986 Jul 25;261(21):9614-21 PMID: 2874131
  28. Location of the 100 kd-50 kd accessory proteins in clathrin coats.
    EMBO J. 1986 Sep;5(9):2079-85 PMID: 2877872
  29. Purification and properties of a new clathrin assembly protein.
    EMBO J. 1986 Dec 1;5(12):3143-9 PMID: 3816757
  30. Receptor-mediated endocytosis: concepts emerging from the LDL receptor system.
    Annu Rev Cell Biol. 1985;1:1-39 PMID: 2881559
  31. 100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies.
    J Cell Biol. 1987 Apr;104(4):887-95 PMID: 2881934
  32. Analysis of 100 kDa polypeptides from coated vesicles of bovine brain: two-dimensional tryptic and chymotryptic maps.
    Biochim Biophys Acta. 1987 May 19;924(2):303-11 PMID: 2882782
  33. Phosphorylation characteristics of brain clathrin-coated vesicle endogenous proteins.
    J Neurochem. 1987 Aug;49(2):434-41 PMID: 2885397
  34. Clathrin beta-light chain of rat liver coated vesicles is phosphorylated in vitro and in vivo.
    FEBS Lett. 1987 Aug 10;220(1):143-8 PMID: 2886368
  35. Biosynthetic protein transport and sorting by the endoplasmic reticulum and Golgi.
    Annu Rev Biochem. 1987;56:829-52 PMID: 3304148
  36. Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein.
    J Biol Chem. 1977 Sep 25;252(18):6510-5 PMID: 893422
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-04-00
Pages
919-29
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454417
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2