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PMID: 6146630 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An enzyme that removes clathrin coats: purification of an uncoating ATPase.

The Journal of cell biology ·Vol. 99 ·No. 2 ·1984-08-00 ·Pages 723-33

Schlossman DM, Schmid SL, Braell WA, Rothman JE

Abstract

Uncoating ATPase, an abundant 70,000-mol-wt polypeptide mediating the ATP-dependent dissociation of clathrin from coated vesicles and empty clathrin cages, has been purified to virtual homogeneity from calf brain cytosol. Uncoating protein is present in cells in amounts roughly stoichiometric with clathrin. This enzyme is isolated as a mixture of monomers and dimers, both forms being active. ATP can support protein-facilitated dissociation of clathrin at micromolar levels; all other ribotriphosphates as well as deoxy-ATP are inactive. The clathrin that is released from cages consists of trimers (triskelions) in a stoichiometric complex with uncoating ATPase. These complexes with clathrin have little tendency to self-associate at neutral pH, and at acidic pH they interfere with the assembly of free clathrin. The possible existence and function of these complexes as clathrin carriers in cells would explain why uncoating protein is made in quantities equivalent to clathrin.

MeSH Terms
Adenosine Triphosphatases/isolation & purification,metabolism Animals Brain/enzymology Cations, Divalent Cations, Monovalent Cattle Cell Fractionation Clathrin/metabolism Coated Pits, Cell-Membrane/ultrastructure Cytosol/enzymology Endosomes/ultrastructure Kinetics Molecular Weight Osmolar Concentration Substrate Specificity Tritium
Chemicals
Cations, Divalent Cations, Monovalent Clathrin Tritium Adenosine Triphosphatases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schlossman D M
Schmid S L
Braell W A
Rothman J E
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44 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.99.2.723
Published
1984-08-00
Pages
723-33
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113251
Subset
IM
Grants
NIGMS NIH HHS · GM 25662 · United States
External Links
PubMed source DOI: 10.1083/jcb.99.2.723 PMC Full Text View journal details
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