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PMID: 34148 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Formation and properties of 1000-A-diameter, single-bilayer phospholipid vesicles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 1 ·1979-01-00 ·Pages 145-9

Enoch HG, Strittmatter P

Abstract

Two methods are reported for the formation of large, uniform-sized phospholipid vesicles. The methods involve the treatment of phospholipid, in the form of either small, sonicated vesicles or a dry lipid film, at a molar ratio of deoxycholate to phospholipid of 1:2. Subsequent removal of deoxycholate yields a stable preparation of vesicles. These vesicles are bounded by a single bilayer, have an average diameter of 1000 A, and are readily separated from sonicated vesicles (230 A) by gel filtration on Sepharose 4B. Since the 1000-A vesicles are capable of trapping enzymes and other macromolecules, they may prove valuable for the delivery of liposome-entrapped solutes to cells and for the localization of peptide segments of a spectrum of membrane-bound proteins.

MeSH Terms
Bile Acids and Salts Chromatography, Gel/methods Hydrogen-Ion Concentration Liposomes/chemical synthesis Membrane Lipids Microscopy, Electron Molecular Weight Phosphatidylcholines Phospholipids Salts
Chemicals
Bile Acids and Salts Liposomes Membrane Lipids Phosphatidylcholines Phospholipids Salts
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Enoch H G
Strittmatter P
References (27)
27 references, click to expand
  1. Single bilayer vesicles prepared without sonication. Physico-chemical properties.
    Biochim Biophys Acta. 1976 Dec 2;455(2):322-31 PMID: 1033769
  2. Solubilization of membranes by detergents.
    Biochim Biophys Acta. 1975 Mar 25;415(1):29-79 PMID: 1091302
  3. A calorimetric and fluorescent probe study of the gel-liquid crystalline phase transition in small, single-lamellar dipalmitoylphosphatidylcholine vesicles.
    Biochemistry. 1976 Apr 6;15(7):1393-401 PMID: 1259944
  4. The nature of the heme binding in microsomal cytochrome b5.
    J Biol Chem. 1960 Aug;235:2492-7 PMID: 13835239
  5. CHROMATOGRAPHICALLY HOMOGENEOUS LECITHIN FROM EGG PHOSPHOLIPIDS.
    J Am Oil Chem Soc. 1965 Jan;42:53-6 PMID: 14228472
  6. Geometric packing constraints in egg phosphatidylcholine vesicles.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):308-10 PMID: 272647
  7. Purification of stearyl-CoA desaturase from liver.
    Methods Enzymol. 1978;52:188-93 PMID: 27698
  8. Incorporation of macromolecules within large unilamellar vesicles (LUV).
    Ann N Y Acad Sci. 1978;308:259-67 PMID: 279293
  9. The introduction of enzymes into cells by means of liposomes.
    J Lipid Res. 1978 Mar;19(3):289-303 PMID: 349106
  10. Single bilayer liposomes.
    Biochim Biophys Acta. 1971 Jun 1;233(3):820-6 PMID: 4107123
  11. Endycytosis and exocytosis: role of microfilaments and involvement of phospholipids in membrane fusion.
    J Supramol Struct. 1974;2(5-6):517-28 PMID: 4376822
  12. Procedure for the selective release of content from microsomal vesicles without membrane disassembly.
    Methods Enzymol. 1974;31:215-25 PMID: 4419291
  13. Carboxypeptidase from yeast. Large scale preparation and the application to COOH-terminal analysis of peptides and proteins.
    J Biol Chem. 1973 Apr 10;248(7):2296-302 PMID: 4572727
  14. Single bilayer liposomes prepared without sonication.
    Biochim Biophys Acta. 1973 Apr 16;298(4):1015-9 PMID: 4738145
  15. The characterization and interconversions of two conformational states of cytochrome b5 reductase.
    J Biol Chem. 1971 Feb 25;246(4):1017-24 PMID: 5543666
  16. Interactions of basic proteins with phospholipid membranes. Binding and changes in the sodium permeability of phosphatidylserine vesicles.
    J Biol Chem. 1971 Feb 25;246(4):1142-8 PMID: 5543681
  17. Phospholipid spherules (liposomes) as a model for biological membranes.
    J Lipid Res. 1968 May;9(3):310-8 PMID: 5646182
  18. Studies on phosphatidylcholine vesicles. Formation and physical characteristics.
    Biochemistry. 1969 Jan;8(1):344-52 PMID: 5777332
  19. The biophysics of lipidic associations. I. The ternary systems: lecithin-bile salt-water.
    Biochim Biophys Acta. 1966 Dec 7;125(3):563-80 PMID: 5981996
  20. The preparation of large single bilayer liposomes by a fast and controlled dialysis.
    Biochim Biophys Acta. 1978 Sep 11;512(1):147-55 PMID: 698210
  21. New aspects of liposomes.
    Biochim Biophys Acta. 1976 Dec 14;457(3-4):259-302 PMID: 793635
  22. Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles.
    Biochim Biophys Acta. 1975 Jul 3;394(3):483-91 PMID: 805602
  23. Rat liver proteins capable of transferring phosphatidylethanolamine. Purification and transfer activity for other phospholipids and cholesterol.
    J Biol Chem. 1977 Mar 10;252(5):1613-9 PMID: 838732
  24. Exchange of cytochrome b5 between phospholipid vesicles.
    J Biol Chem. 1977 Jul 25;252(14):4842-9 PMID: 873919
  25. Cytochrome b5 and cytochrome b5 reductase-phospholipid vesicles. Intervesicle protein transfer and oreintation factors in protein-protein interactions.
    J Biol Chem. 1977 Aug 25;252(16):5656-60 PMID: 885870
  26. Large volume liposomes by an ether vaporization method.
    Biochim Biophys Acta. 1976 Sep 7;443(3):629-34 PMID: 963074
  27. Fluorescence depolarization studies of phase transitions and fluidity in phospholipid bilayers. 1. Single component phosphatidylcholine liposomes.
    Biochemistry. 1976 Oct 5;15(20):4521-8 PMID: 974073
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.1.145
Published
1979-01-00
Pages
145-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC382893
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.1.145 PMC Full Text View journal details
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