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PMID: 3419922 Published · ppublish English Journal Article

The leading sequence of caulimovirus large RNA can be folded into a large stem-loop structure.

Nucleic acids research ·Vol. 16 ·No. 17 ·1988-09-12 ·Pages 8377-90

Fütterer J, Gordon K, Bonneville JM, Sanfaçon H, Pisan B, Penswick J, Hohn T

Abstract

The 600 nt long sequences preceeding the first large ORFs (ORF VII) of three caulimoviruses, although varying in primary sequence, can be folded into a large stem/loop structure centered around a conserved stretch of 36 nucleotides. Deletions of the conserved sequence delay symptom appearance considerably, but do not affect expression of a reporter gene in plant protoplasts. Another striking similarity between the leaders concerns the number and distribution of small open reading frames (sORF) they carry. Expression of two of these sORFs was tested by fusion of a reporter gene: both were expressed in plant protoplasts.

MeSH Terms
Base Sequence Enhancer Elements, Genetic Genes, Viral Models, Molecular Molecular Sequence Data Nucleic Acid Conformation Plant Viruses/genetics Promoter Regions, Genetic RNA, Viral/genetics,ultrastructure Retroviridae/genetics Software
Chemicals
RNA, Viral
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Fütterer J
Friedrich Miescher-Institut, Basel, Switzerland.
Gordon K
Bonneville J M
Sanfaçon H
Pisan B
Penswick J
Hohn T
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.17.8377
Published
1988-09-12
Pages
8377-90
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338565
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.17.8377 PMC Full Text View journal details
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