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PMID: 3439797 Published · ppublish English Journal Article

Phenotypic expression and genetic heterogeneity of lincosamide inactivation in Staphylococcus spp.

Antimicrobial agents and chemotherapy ·Vol. 31 ·No. 12 ·1987-12-00 ·Pages 1887-91

Leclercq R, Brisson-Noël A, Duval J, Courvalin P

Abstract

We examined the resistance phenotype and the genetic basis of lincosamide modification in 25 clinical isolates of Staphylococcus spp. inactivating lincomycin and clindamycin. The strains were resistant to high levels of lincomycin but remained susceptible to clindamycin. However, MBCs and inoculum effects showed that the activity of clindamycin was impaired. The distribution in these strains of nucleotide sequences related to linA and linA', the genes encoding lincosamide nucleotidylation in Staphylococcus haemolyticus BM4610 and S. aureus BM4611, respectively, was studied by dot blot hybridization. The genes responsible for lincosamide inactivation in Staphylococcus spp. were found to constitute a family of related sequences which are not species specific.

MeSH Terms
Anti-Bacterial Agents/metabolism Culture Media DNA, Bacterial/biosynthesis Drug Resistance, Microbial Lincosamides Macrolides Nucleic Acid Hybridization Phenotype Staphylococcus/genetics,metabolism
Chemicals
Anti-Bacterial Agents Culture Media DNA, Bacterial Lincosamides Macrolides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Leclercq R
Service de Bactériologie-Virologie, Hôpital Henri Mondor, Université Paris XII, Créteil, France.
Brisson-Noël A
Duval J
Courvalin P
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.31.12.1887
Published
1987-12-00
Pages
1887-91
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC175821
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.31.12.1887 PMC Full Text View journal details
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