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PMID: 3023281 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Complete nucleotide sequence and transcription of ermF, a macrolide-lincosamide-streptogramin B resistance determinant from Bacteroides fragilis.

Journal of bacteriology ·Vol. 168 ·No. 2 ·1986-11-00 ·Pages 523-33

Rasmussen JL, Odelson DA, Macrina FL

Abstract

DNA sequence analysis of a portion of an EcoRI fragment of the Bacteroides fragilis R plasmid pBF4 has allowed us to identify the macrolide-lincosamide-streptogramin B resistance (MLSr) gene, ermF. ermF had a relative moles percent G + C of 32, was 798 base pairs in length, and encoded a protein of approximately 30,360 daltons. Comparison between the deduced amino acid sequence of ermF and six other erm genes from gram-positive bacteria revealed striking homologies among all of these determinants, suggesting a common origin. Based on these and other data, we believe that ermF codes for an rRNA methylase. Analysis of the nucleotide sequences upstream and downstream from the ermF gene revealed the presence of directly repeated sequences, now identified as two copies of the insertion element IS4351. One of these insertion elements was only 26 base pairs from the start codon of ermF and contained the transcriptional start signal for this gene as judged by S1 nuclease mapping experiments. Additional sequence analysis of the 26 base pairs separating ermF and IS4351 disclosed strong similarities between this region and the upstream regulatory control sequences of ermC and ermA (determinants of staphylococcal origin). These results suggested that ermF was not of Bacteroides origin and are discussed in terms of the evolution of ermF and the expression of drug resistance in heterologous hosts.

MeSH Terms
Amino Acid Sequence Anti-Bacterial Agents/pharmacology Bacteroides fragilis/drug effects,genetics Base Sequence DNA Transposable Elements DNA, Bacterial Gene Expression Regulation Genes, Bacterial Lincosamides Macrolides R Factors Transcription, Genetic Virginiamycin/pharmacology
Chemicals
Anti-Bacterial Agents DNA Transposable Elements DNA, Bacterial Lincosamides Macrolides Virginiamycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rasmussen J L
Odelson D A
Macrina F L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.168.2.523-533.1986
Published
1986-11-00
Pages
523-33
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213512
Subset
IM
Grants
NIAID NIH HHS · AI20153 · United States
Databases
GENBANK
M14730
External Links
PubMed source DOI: 10.1128/jb.168.2.523-533.1986 PMC Full Text View journal details
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