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PMID: 6429478 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Evolutionary relationships of the Bacillus licheniformis macrolide-lincosamide-streptogramin B resistance elements.

Molecular & general genetics : MGG ·Vol. 194 ·No. 3 ·1984-00-00 ·Pages 362-7

Israeli-Reches M, Weinrauch Y, Dubnau D

Abstract

Naturally occurring erythromycin (Em) resistance was found in 11 of the 18 Bacillus licheniformis isolates tested but was absent from a wide variety of other Bacillus strains. The Em resistance elements confer inducible macrolide-lincosamide-streptogramin B (MLS) resistance and are related to ermD , an MLS resistance element previously cloned from the chromosome of B. licheniformis 749. The MLS sensitive B. licheniformis strains and the other sensitive Bacillus strains tested, lack sequences with detectable homology to ermD . The sensitive B. licheniformis strains do exhibit homology to sequences which flank ermD in B. licheniformis 749. The relative sizes of the homologous DNA fragments suggest that the sensitive strains are lacking a 3.6 kb segment which contains ermD . It is shown that ermD is homologous to chromosomal DNA from Streptomyces erythreus ATCC 11635, an Em producing organism. These observations suggest to us that MLS resistance may have arisen in the Streptomyces and spread to B. licheniformis, another gram positive bacterium found in soil. It is further proposed that ermD is or was located on a transposon-like element and has spread and evolved further to yield a variety of related Staphylococcal and Streptococcal MLS determinants.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacillus/drug effects,genetics Bacterial Proteins/genetics Biological Evolution DNA, Bacterial/genetics Drug Resistance, Microbial Erythromycin/pharmacology Lincosamides Macrolides R Factors Species Specificity Streptomyces/genetics Virginiamycin/pharmacology tRNA Methyltransferases/genetics
Chemicals
Anti-Bacterial Agents Bacterial Proteins DNA, Bacterial Lincosamides Macrolides Virginiamycin Erythromycin tRNA Methyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Israeli-Reches M
Weinrauch Y
Dubnau D
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35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425545
Published
1984-00-00
Pages
362-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425545
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