Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA.

Journal of bacteriology ·Vol. 164 ·No. 1 ·1985-10-00 ·Pages 466-9

Smith CJ

Abstract

A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for the preparation of frozen competent cells is also described.

MeSH Terms

Bacteroides fragilis/genetics,growth & development DNA, Bacterial Hydrogen-Ion Concentration Plasmids Polyethylene Glycols/pharmacology Temperature Transformation, Bacterial/drug effects

Chemicals

DNA, Bacterial Polyethylene Glycols

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Smith C J

References (15)

15 references, click to expand

Calcium-dependent bacteriophage DNA infection.
J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
Human fecal flora: the normal flora of 20 Japanese-Hawaiians.
Appl Microbiol. 1974 May;27(5):961-79 PMID: 4598229
Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
Effect of diet on human fecal flora: comparison of Japanese and American diets.
Am J Clin Nutr. 1974 Dec;27(12):1456-69 PMID: 4432829
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.
Mol Gen Genet. 1979 Jan 5;168(1):111-5 PMID: 107388
Transfer of multiple antibiotic resistance between subspecies of Bacteroides fragilis.
J Infect Dis. 1979 Jan;139(1):97-101 PMID: 108340
Genetic transformation of Rhizobium meliloti by plasmid DNA.
Gene. 1981 Nov;15(2-3):279-83 PMID: 7297855
Transformation of Rhizobium meliloti 41 with plasmid DNA.
J Bacteriol. 1982 May;150(2):465-70 PMID: 6279558
Two independent conjugal transfer systems operating in Bacteroides fragilis V479-1.
J Bacteriol. 1982 Jul;151(1):281-7 PMID: 7085560
Characterization of pBFTM10, a clindamycin-erythromycin resistance transfer factor from Bacteroides fragilis.
J Bacteriol. 1982 Aug;151(2):686-91 PMID: 7096266
Genetic transformation of Rhodopseudomonas sphaeroides by plasmid DNA.
J Bacteriol. 1982 Oct;152(1):89-97 PMID: 6981642
Studies on transformation of Escherichia coli with plasmids.
J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast.
Gene. 1983 Nov;25(2-3):333-41 PMID: 6363214
Plasmid transfer from Escherichia coli to Bacteroides fragilis: differential expression of antibiotic resistance phenotypes.
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7203-6 PMID: 6095273
Characterization of Bacteroides ovatus plasmid pBI136 and structure of its clindamycin resistance region.
J Bacteriol. 1985 Mar;161(3):1069-73 PMID: 2982783

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.164.1.466-469.1985

Published

1985-10-00

Pages

466-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC214267

Subset

External Links

PubMed source DOI: 10.1128/jb.164.1.466-469.1985 PMC Full Text View journal details

Analysis Services

Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA.

Contact

WeChat Official Account