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PMID: 3447741 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The watermelon mitochondrial URF-1 gene: evidence for a complex structure.

Current genetics ·Vol. 10 ·No. 11 ·1986-00-00 ·Pages 857-69

Stern DB, Bang AG, Thompson WF

Abstract

We have cloned and sequenced a fragment of watermelon mitochondrial DNA (mtDNA) which contains a gene homologous to mitochondrial URF-1 (Unidentified Reading Frame-1) of vertebrates, Drosophila yakuba and Aspergillus nidulans. URF-1 is thought to encode a component of the respiratory chain NADH dehydrogenase. Two coding regions in the watermelon gene are separated by approximately 1,450 bp of untranslatable DNA. These two exons encode the central portions of URF-1, and are highly conserved. We postulate that three additional exons, selected by their map location and amino acid homology to other URF-1 sequences, encode the remainder of the polypeptide. This is the first description of a plant mitochondrial gene with multiple introns.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Codon/genetics DNA, Mitochondrial/genetics Exons Genes Introns Molecular Sequence Data Plant Proteins/genetics Plants/genetics Transcription, Genetic
Chemicals
Codon DNA, Mitochondrial Plant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stern D B
Carnegie Institution of Washington, Department of Plant Biology, Stanford, CA 94305.
Bang A G
Thompson W F
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00418532
Published
1986-00-00
Pages
857-69
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Databases
GENBANK
X04130
External Links
PubMed source DOI: 10.1007/BF00418532 View journal details
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