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PMID: 3870928 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mitochondrial gene expression in Cucurbitaceae: conserved and variable features.

Current genetics ·Vol. 9 ·No. 5 ·1985-00-00 ·Pages 395-404

Stern DB, Newton KJ

Abstract

We have examined mitochondrial DNA (mtDNA) sequence conservation, transcriptional patterns of mitochondrial genes, and mitochondrial translation products in four species of the plant family Cucurbitaceae, in which there is a seven-fold range in mitochondrial genome size. A set of conserved mtDNA sequences which we term "core" DNA is present in all cucurbit genomes examined. In watermelon, only those mtDNA restriction fragments which contain "core" DNA hybridize with mtRNA. Similar numbers of polypeptides are synthesized by isolated mitochondria from all species examined, but an extra set of mtDNA sequences is transcribed in the largest genome (muskmelon). Taken together, these results suggest that some mtRNA is untranslated in muskmelon. Cloned mitochondrial genes of known function, from maize, identified mitochondrial transcripts that varied in both size and number among cucurbit species.

MeSH Terms
Base Sequence Mitochondria/ultrastructure Plant Proteins/genetics Plants/genetics Protein Biosynthesis Transcription, Genetic
Chemicals
Plant Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stern D B
Carnegie Institution of Washington, Department of Plant Biology, Stanford, California 94305.
Newton K J
References (41)
41 references, click to expand
  1. Variation in mitochondrial translation products associated with male-sterile cytoplasms in maize.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3841-5 PMID: 16592554
  2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  3. Sequence and organization of the human mitochondrial genome.
    Nature. 1981 Apr 9;290(5806):457-65 PMID: 7219534
  4. The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons.
    Cell. 1981 Nov;26(3 Pt 1):315-23 PMID: 6276012
  5. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  6. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801-5 PMID: 368799
  7. The alpha-subunit of the maize F(1)-ATPase is synthesised in the mitochondrion.
    EMBO J. 1983;2(10):1783-9 PMID: 16453476
  8. Recombination sequences in plant mitochondrial genomes: diversity and homologies to known mitochondrial genes.
    Nucleic Acids Res. 1984 Aug 10;12(15):6141-57 PMID: 6473104
  9. Nuclear and cytoplasmic genes controlling synthesis of variant mitochondrial polypeptides in male-sterile maize.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):418-22 PMID: 16592757
  10. Synthesis of a dicyclohexylcarbodiimide-binding proteolipid by cucumber (Cucumis sativus L.) mitochondria.
    Curr Genet. 1984 Sep;8(7):537-42 PMID: 24177955
  11. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  12. The apocytochrome b gene in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site.
    EMBO J. 1984 Sep;3(9):2107-13 PMID: 16453553
  13. Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome.
    J Mol Biol. 1982 Apr 25;156(4):683-717 PMID: 7120390
  14. Mapping the mitochondrial DNA of Zea mays: Ribosomal gene localization.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):5926-9 PMID: 16593234
  15. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  16. The mitochondrial genome is large and variable in a family of plants (cucurbitaceae).
    Cell. 1981 Sep;25(3):793-803 PMID: 6269758
  17. Maize mitochondrial DNA contains a sequence homologous to the ribulose-1,5-bisphosphate carboxylase large subunit gene of chloroplast DNA.
    Cell. 1983 Oct;34(3):1007-14 PMID: 6354467
  18. Organization and expression of the mitochondrial genome of plants I. The genes for wheat mitochondrial ribosomal and transfer RNA: evidence for an unusual arrangement.
    Nucleic Acids Res. 1980 Jan 25;8(2):319-35 PMID: 6252544
  19. Evidence for homologous recombination between repeated sequences containing 18S and 5S ribosomal RNA genes in wheat mitochondrial DNA.
    EMBO J. 1984 Feb;3(2):297-302 PMID: 16453499
  20. The tRNA genes punctuate the reading of genetic information in human mitochondrial DNA.
    Cell. 1980 Nov;22(2 Pt 2):393-403 PMID: 7448867
  21. Phytochrome control of RNA levels in developing pea and mung-bean leaves.
    Planta. 1983 Aug;158(6):487-500 PMID: 24264922
  22. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit.
    Cell. 1976 Nov;9(3):431-8 PMID: 1033038
  23. Chloroplast DNA rearrangements are more frequent when a large inverted repeat sequence is lost.
    Cell. 1982 Jun;29(2):537-50 PMID: 6288261
  24. Extensive and widespread homologies between mitochondrial DNA and chloroplast DNA in plants.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1946-50 PMID: 16593442
  25. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  26. Physical and gene mapping of chloroplast DNA from Atriplex triangularis and Cucumis sativa.
    Nucleic Acids Res. 1982 Mar 11;10(5):1593-605 PMID: 6280152
  27. Molecular integrity of chloroplast ribosomal ribonucleic acid.
    Biochem J. 1973 Sep;135(1):237-40 PMID: 4776871
  28. Sequence and gene organization of mouse mitochondrial DNA.
    Cell. 1981 Oct;26(2 Pt 2):167-80 PMID: 7332926
  29. Cytochrome oxidase subunit II gene in mitochondria of Oenothera has no intron.
    EMBO J. 1983;2(12):2173-8 PMID: 16453484
  30. Pronounced structural similarities between the small subunit ribosomal RNA genes of wheat mitochondria and Escherichia coli.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):493-7 PMID: 6364144
  31. Plant mitochondrial nucleic acids.
    Biochem Soc Symp. 1973;(38):175-93 PMID: 4807458
  32. Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolation.
    Nucleic Acids Res. 1977 Sep;4(9):3055-64 PMID: 410006
  33. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  34. Transcription maps of mtDNAs of two strains of saccharomyces: transcription of strain-specific insertions; Complex RNA maturation and splicing.
    Cell. 1979 Oct;18(2):511-23 PMID: 227609
  35. The mitochondrial genome of yeast.
    Cell. 1978 Nov;15(3):705-23 PMID: 153200
  36. Organization of the mitochondrial ribosomal RNA genes of maize.
    Nucleic Acids Res. 1982 Jun 11;10(11):3333-40 PMID: 6285296
  37. Physicochemical characterization of mitochondrial DNA from pea leaves.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1830-4 PMID: 4505661
  38. The Relationship between Satellite Deoxyribonucleic Acid, Ribosomal Ribonucleic Acid Gene Redundancy, and Genome Size in Plants.
    Plant Physiol. 1975 Mar;55(3):496-501 PMID: 16659109
  39. Mitochondrial and chloroplast genomes of maize have a 12-kilobase DNA sequence in common.
    Nature. 1982 Oct 21;299(5885):698-702 PMID: 6889685
  40. Sequence analysis of the maize mitochondrial 26 S rRNA gene and flanking regions.
    Plasmid. 1984 Mar;11(2):141-50 PMID: 6728934
  41. Conservation of ribosomal RNA gene arrangement in the mitochondrial DNA of angiosperms.
    Plant Mol Biol. 1982 Sep;1(3):245-9 PMID: 24317966
Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00421611
Published
1985-00-00
Pages
395-404
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Grants
NIGMS NIH HHS · GM29975 · United States
External Links
PubMed source DOI: 10.1007/BF00421611 View journal details
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