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PMID: 345100 Published · ppublish English Journal Article

Construction and properties of recombinant plasmids containing the rII genes of bacteriophage T4.

Molecular & general genetics : MGG ·Vol. 159 ·No. 3 ·1978-02-27 ·Pages 301-9

Selzer G, Bolle A, Krisch B, Epstein R

Abstract

The EcoRI digestion products of phage T4 DNA have been examined using a phage DNA transformation assay. A 2.6 X 10(6) Dalton fragment was found to contain the rII genes. This fragment was purified and then treated with HindIII endonuclease. The cleavage products were ligated to the vector plasmid pBR313 and viable recombinant plasmids recovered. A genetic assay was employed to demonstrate that the recombinants contained T4 DNA and to localize on the phage genetic map the EcoRI and HindIII sites cleaved during the construction of the plasmids. Preliminary characterization suggests that a fragment covering the beginning of the rIIA gene possibly contains a promotor which is active in uninfected cells.

MeSH Terms
Cell Transformation, Viral Coliphages/genetics DNA Restriction Enzymes/metabolism DNA, Bacterial DNA, Recombinant DNA, Viral/analysis,isolation & purification Escherichia coli/genetics Genes, Viral Genetic Linkage Plasmids
Chemicals
DNA, Bacterial DNA, Recombinant DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Selzer G
Bolle A
Krisch B
Epstein R
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22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268267
Published
1978-02-27
Pages
301-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00268267
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