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PMID: 4847325 Published · ppublish English Journal Article

Point mutants in the D2a region of bacteriophage T4 fail to induce T4 endonuclease IV.

Journal of virology ·Vol. 14 ·No. 2 ·1974-08-00 ·Pages 207-13

Vetter D, Sadowski PD

Abstract

We have studied the properties of presumptive point mutants in the D2a region of bacteriophage T4. Dominance tests showed that the D2a mutation was recessive to the wild-type allele. The mutations were shown to map in the D2a region by complementation against rII deletions. The D2a mutations were also located between gene 52 and rIIB by two- and three-factor crosses. The mutants are located at at least two distinct loci in the D2a region. The point mutants grow normally on all hosts tested and none of the mutants makes T4 endonuclease IV. We propose the name "denB" for the D2a locus.

MeSH Terms
Coliphages/enzymology,growth & development,metabolism Crosses, Genetic DNA, Viral/biosynthesis Deoxyribonucleases/biosynthesis Endonucleases/biosynthesis Enzyme Induction Genes Genetic Complementation Test Lysogeny Mutation Thymidine/metabolism Tritium Virus Replication
Chemicals
DNA, Viral Tritium Deoxyribonucleases Endonucleases Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vetter D
Sadowski P D
References (23)
23 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.14.2.207-213.1974
Published
1974-08-00
Pages
207-13
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355505
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.14.2.207-213.1974 PMC Full Text View journal details
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