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PMID: 3455774 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Introduction of genes into preimplantation mouse embryos by use of a defective recombinant retrovirus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 2 ·1986-01-00 ·Pages 366-8

Rubenstein JL, Nicolas JF, Jacob F

Abstract

Two-cell and four-cell preimplantation mouse embryos were cocultured in vitro with fibroblasts producing the recombinant retrovirus M-MuLV-neo. No wildtype helper virus was detected in these cultures. Of the embryos that survived the in vitro cultivation and the reimplantation into foster mothers, 2 of 15 that were tested contained the proviral genome. The provirus integrated as a single copy at a unique site. We estimate that approximately equal to 20% of the cells in each of the two transgenic fetuses contained the provirus.

MeSH Terms
Animals Cleavage Stage, Ovum Defective Viruses/genetics Drug Resistance Embryonic Development Female Genes, Bacterial Genetic Engineering/methods Genetic Vectors Mice/embryology Moloney murine leukemia virus/genetics Mosaicism Neomycin/pharmacology Pregnancy Transfection
Chemicals
Neomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rubenstein J L
Nicolas J F
Jacob F
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.2.366
Published
1986-01-00
Pages
366-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322859
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.2.366 PMC Full Text View journal details
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