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PMID: 3456150 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Promoter and enhancer activities of long terminal repeats associated with cellular retrovirus-like (VL30) elements.

Nucleic acids research ·Vol. 14 ·No. 2 ·1986-01-24 ·Pages 645-58

Rotman G, Itin A, Keshet E

Abstract

LTR units associated with cellular retrovirus-like elements are abundantly present in chromosomal DNA of animal cells. We have analyzed the promoter and enhancer activities of diverse LTR units associated with different members of the murine retrovirus-like family known as VL30. We report here that the structurally heterogenous VL30 LTRs displayed highly variable promoter/enhancer activities. The most active VL30 LTR (designated VL3) promoted CAT activity to levels six-fold higher than the LTR of the strongly transforming retrovirus, MSV. This VL30 transcription unit, containing a unique U3 region, was further characterized by S1 nuclease mapping. VL3 LTR functioned as an enhancer in CAT constructs containing a SV40 promoter. In addition, a defined U3 segment was shown to augment expression of CAT in an orientation independent manner. VL3 LTR also served as an efficient promoter and enhancer in heterologous monkey cells. These results suggest that certain resident LTRs possess promoter and enhancer capacities greater than those possessed by LTRs of infectious retroviruses.

MeSH Terms
Acetyltransferases/genetics Animals Cell Line Chloramphenicol O-Acetyltransferase Chlorocebus aethiops Cloning, Molecular Enhancer Elements, Genetic Genes Genes, Regulator Genes, Viral Kidney L Cells/enzymology Mice Mice, Inbred BALB C Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid Retroviridae/genetics Transcription, Genetic Transfection
Chemicals
Acetyltransferases Chloramphenicol O-Acetyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rotman G
Itin A
Keshet E
References (21)
21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.2.645
Published
1986-01-24
Pages
645-58
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339455
Subset
IM
Databases
GENBANK
X03489
External Links
PubMed source DOI: 10.1093/nar/14.2.645 PMC Full Text View journal details
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