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PMID: 3456614 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Growth requirements in vitro of oligodendrocyte cell lines and neonatal rat brain oligodendrocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 6 ·1986-03-00 ·Pages 1955-9

Bottenstein JE

Abstract

I have defined the basic requirements for the proliferation of cell lines expressing oligodendrocyte properties and for the survival of galactocerebroside-positive oligodendrocytes derived from neonatal rat brains. Conventional serum-containing medium can be replaced by 01 medium, a chemically defined medium supplemented with insulin, transferrin, sodium selenite, and biotin. Thyroid hormone is not required. When cells are plated directly into O1 medium, the substratum has to be modified by precoating with polylysine and adding fibronectin to the medium prior to the cells. Both cell lines and brain cells can be subcultured numerous times in O1 medium without initial culture in serum-containing medium. Brain cultures can be maintained in O1 medium for several months and contain a significantly higher percentage of mature oligodendrocytes, a lower number of astrocytes, and no fibroblasts as compared to cells maintained in serum-containing medium.

MeSH Terms
Animals Animals, Newborn Brain/cytology Cell Differentiation Cell Division Cell Survival Cells, Cultured Culture Media/analysis Culture Techniques/methods Growth Substances/metabolism Hormones/metabolism Neuroglia/metabolism Oligodendroglia/cytology,metabolism Rats Selenium/metabolism Transferrin/metabolism
Chemicals
Culture Media Growth Substances Hormones Transferrin Selenium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Bottenstein J E
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.6.1955
Published
1986-03-00
Pages
1955-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323203
Subset
IM
Grants
NINDS NIH HHS · NS 20375 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.6.1955 PMC Full Text View journal details
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