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PMID: 3459154 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Complete amino acid sequence of chicken cartilage link protein deduced from cDNA clones.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 11 ·1986-06-00 ·Pages 3766-70

Deák F, Kiss I, Sparks KJ, Argraves WS, Hampikian G, Goetinck PF

Abstract

cDNA clones coding for chicken cartilage link protein were isolated and sequenced. The DNA sequence for the entire core polypeptide of the mature link protein and the predicted signal peptide consists of 1065 nucleotides. The deduced primary translation product (355 amino acids) has a molecular mass of 40.7 kDa; the calculated molecular mass of the mature link protein core polypeptide (340 amino acids) is 39.06 kDa. The DNA sequence contains two tandemly arranged repeat sequences that may code for repeated functional domains of link protein involved in binding to hyaluronic acid. The mRNAs for chicken link protein are 6.0, 5.8, and 3.0 kilobase pairs, and the difference between the sizes of the RNA species lies in the 3' untranslated region.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cartilage/physiology Chickens Cloning, Molecular DNA/genetics Extracellular Matrix Proteins Proteins/genetics Proteoglycans RNA, Messenger/genetics Sequence Homology, Nucleic Acid Solubility
Chemicals
Extracellular Matrix Proteins Proteins Proteoglycans RNA, Messenger link protein DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Deák F
Kiss I
Sparks K J
Argraves W S
Hampikian G
Goetinck P F
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.11.3766
Published
1986-06-00
Pages
3766-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323604
Subset
IM
Grants
NICHD NIH HHS · HD 09174 · United States
Databases
GENBANK
M13212
External Links
PubMed source DOI: 10.1073/pnas.83.11.3766 PMC Full Text View journal details
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