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PMID: 3459708 Published · ppublish English Comparative Study Journal Article

DNA sequence of the coding region of the HLA-B44 gene.

Immunogenetics ·Vol. 23 ·No. 6 ·1986-00-00 ·Pages 396-400

Kottmann AH, Seemann GH, Guessow HD, Roos MH

Abstract

An HLA-B44 cDNA clone was identified in a cDNA library constructed from an HLA-B44 homozygous cell line. The DNA sequence was determined and was found to contain the complete coding sequence but for (probably) the three N-terminal codons. Comparisons of the derived amino acid sequence with other HLA-A and -B locus amino acid sequences revealed four HLA-B44-specific substitutions including a new polymorphic site. Regions of strong sequence conservation for HLA-B-locus products were found at the nucleotide and amino acid levels.

MeSH Terms
Amino Acid Sequence Base Sequence DNA/analysis Genes HLA Antigens/genetics HLA-A Antigens HLA-B Antigens HLA-B44 Antigen Humans Polymorphism, Genetic
Chemicals
HLA Antigens HLA-A Antigens HLA-B Antigens HLA-B44 Antigen DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kottmann A H
Seemann G H
Guessow H D
Roos M H
References (31)
31 references, click to expand
  1. Nomenclature for factors of the HLA system 1984.
    Immunogenetics. 1984;20(6):593-601 PMID: 6595218
  2. The complete primary structure of HLA-Bw58.
    J Biol Chem. 1985 Oct 5;260(22):11924-33 PMID: 2995352
  3. Cloning and complete sequence of an HLA-A2 gene: analysis of two HLA-A alleles at the nucleotide level.
    J Immunol. 1985 Apr;134(4):2727-33 PMID: 2982951
  4. Class I-like HLA genes map telomeric to the HLA-A2 locus in human cells.
    Nature. 1983 Apr 7;302(5908):534-6 PMID: 6300687
  5. Biochemical and cellular identification of HLA-Bw44 subtypes.
    Hum Immunol. 1984 Oct;11(2):69-76 PMID: 6333415
  6. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  7. Structural analysis of the functional sites of class I HLA antigens.
    Immunol Rev. 1985 Jul;85:149-68 PMID: 2412949
  8. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):616-20 PMID: 6165999
  9. Complete nucleotide sequence of a functional class I HLA gene, HLA-A3: implications for the evolution of HLA genes.
    EMBO J. 1984 Apr;3(4):887-94 PMID: 6609814
  10. Synthesis of full length cDNAs from four partially purified oviduct mRNAs.
    J Biol Chem. 1978 Apr 10;253(7):2471-82 PMID: 632280
  11. Gene conversion-like mechanisms may generate polymorphism in human class I genes.
    EMBO J. 1986 Mar;5(3):547-52 PMID: 3011411
  12. Primary structure of papain-solubilized human histocompatibility antigen HLA-B40 (-Bw60). An outline of alloantigenic determinants.
    Biochemistry. 1983 Aug 2;22(16):3961-9 PMID: 6193808
  13. The HLA-AW24 gene: sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes.
    Immunogenetics. 1985;21(5):479-89 PMID: 2987115
  14. Complete nucleotide sequence of a gene encoding a functional human class I histocompatibility antigen (HLA-CW3).
    EMBO J. 1984 Apr;3(4):879-85 PMID: 6609813
  15. Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules.
    Cell. 1981 May;24(2):287-99 PMID: 7016338
  16. Primary structure of papain-solubilized human histocompatibility antigen HLA-B27.
    Biochemistry. 1985 Mar 26;24(7):1733-41 PMID: 2408663
  17. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  18. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.
    J Biol Chem. 1978 Apr 10;253(7):2483-95 PMID: 75887
  19. Structure of crossreactive human histocompatibility antigens HLA-A28 and HLA-A2: possible implications for the generation of HLA polymorphism.
    Proc Natl Acad Sci U S A. 1982 Jun;79(12):3813-7 PMID: 6179086
  20. Isolation of HLA locus-specific DNA probes from the 3'-untranslated region.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5175-8 PMID: 6591185
  21. Genes of the major histocompatibility complex in mouse and man.
    Science. 1983 Nov 18;222(4625):727-33 PMID: 6356354
  22. Monoclonal antibodies against two separate alloantigenic sites of HLA-B40.
    Immunogenetics. 1981;13(6):509-27 PMID: 7026428
  23. Further studies on the epitopes of HLA-B7 defined by murine monoclonal antibodies.
    Hum Immunol. 1986 Jan;15(1):44-67 PMID: 2419285
  24. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  25. Antigenic determinants of the HLA-B7 molecule; Bw6- and B7-specific determinants are spatially separate.
    Immunogenetics. 1983;18(1):1-16 PMID: 6190743
  26. Sequences of human repetitive DNA, non-alpha-globin genes, and major histocompatibility locus genes.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:1079-86 PMID: 6574860
  27. Characterization of the homopolymer tailing reaction catalyzed by terminal deoxynucleotidyl transferase. Implications for the cloning of cDNA.
    J Biol Chem. 1982 Dec 25;257(24):14773-82 PMID: 6294077
  28. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  29. Easy identification of cDNA clones.
    EMBO J. 1983;2(10):1791-4 PMID: 6315402
  30. Exon/intron organization and complete nucleotide sequence of an HLA gene.
    Proc Natl Acad Sci U S A. 1982 Feb;79(3):893-7 PMID: 6461010
  31. Molecular cloning and DNA sequence analysis of genes encoding cytotoxic T lymphocyte-defined HLA-A3 subtypes: the E1 subtype.
    J Immunol. 1985 Oct;135(4):2835-41 PMID: 2993417
Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00372673
Published
1986-00-00
Pages
396-400
Language
English
Region
United States
NLM ID
0420404
Subset
IM
Databases
GENBANK
M15470
External Links
PubMed source DOI: 10.1007/BF00372673 View journal details
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