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PMID: 3466022 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells.

Molecular and cellular biology ·Vol. 6 ·No. 2 ·1986-02-00 ·Pages 703-6

Toneguzzo F, Hayday AC, Keating A

Abstract

The technique of DNA transfer by electroporation was investigated in an effort to evaluate its utility for the identification of developmentally controlled regulatory sequences. Transient and stable gene expression was detected in a variety of lymphoid cell lines subjected to electroporation. No correlation existed between the levels of chloramphenicol acetyltransferase (acetyl-CoA; chloramphenicol 3-O-acetyltransferase, EC 2.3.1.28) expression and stable transfection frequency. In all lymphoid cell lines tested, the simian virus 40 early region was a better promoter than was the Rous sarcoma virus long terminal repeat.

MeSH Terms

Acetyltransferases/genetics Animals Cell Line Chloramphenicol O-Acetyltransferase DNA/genetics Electrochemistry Electrodes Genes Genes, Regulator Humans Kinetics Lymphocytes Mice

Chemicals

DNA Acetyltransferases Chloramphenicol O-Acetyltransferase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Toneguzzo F

Hayday A C

Keating A

References (27)

27 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.6.2.703-706.1986

Published

1986-02-00

Pages

703-6

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC367561

Subset

External Links

PubMed source DOI: 10.1128/mcb.6.2.703-706.1986 PMC Full Text View journal details

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Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells.

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