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PMID: 3466183 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification of a presynaptic membrane protein that mediates a calcium-dependent translocation of acetylcholine.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 23 ·1986-12-00 ·Pages 9226-30

Israël M, Morel N, Lesbats B, Birman S, Manaranche R

Abstract

A protein, which we call "mediatophore," that mediates calcium-dependent release of acetylcholine from proteoliposomes has been purified from the presynaptic plasma membrane. About 250 micrograms of this material was obtained from 500 g of Torpedo marmorata electric organ. Precipitation of the protein and subsequent removal of associated lipids inactivated the protein, which then became water soluble; this permitted evaluation of its Stokes radius (52 A) and its sedimentation coefficient (9.8 +/- 0.75 S) and, hence, an approximate molecular mass of 210 +/- 16 kDa could be determined. PAGE analysis showed that the protein is made of 17-kDa subunits, not linked by disulfide bonds. When this material was observed by electron microscopy after negative staining, the apparently pentameric structures had an average diameter of about 7 nm.

MeSH Terms
Acetylcholine/metabolism Animals Calcium/physiology Carrier Proteins/isolation & purification Hydrogen-Ion Concentration Membrane Proteins/isolation & purification Microscopy, Electron Molecular Weight Nerve Tissue Proteins/isolation & purification Neuromuscular Junction/analysis Torpedo
Chemicals
Carrier Proteins Membrane Proteins Nerve Tissue Proteins mediatophore Acetylcholine Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Israël M
Morel N
Lesbats B
Birman S
Manaranche R
References (31)
31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.23.9226
Published
1986-12-00
Pages
9226-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC387108
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.23.9226 PMC Full Text View journal details
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