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PMID: 3474611 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Regulated expression of multiple chicken erythroid membrane skeletal protein 4.1 variants is governed by differential RNA processing and translational control.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 13 ·1987-07-00 ·Pages 4432-6

Ngai J, Stack JH, Moon RT, Lazarides E

Abstract

Protein 4.1 is an extrinsic membrane protein that facilitates the interaction of spectrin and actin in the erythroid membrane skeleton and exists as several structurally related polypeptides in chickens. The ratio of protein 4.1 variants is developmentally regulated during terminal differentiation of chicken erythroid and lenticular cells. To examine the mechanisms by which multiple chicken protein 4.1 variants are differentially expressed, we have isolated cDNA clones specific for chicken erythroid protein 4.1. We show that a single protein 4.1 gene gives rise to multiple 6.6-kilobase mRNAs by differential RNA processing. Furthermore, the ratios of protein 4.1 mRNAs change during chicken embryonic erythropoiesis. We observe a quantitative difference in variant ratios when protein 4.1 is synthesized in vivo or in a rabbit reticulocyte lysate in vitro. Our results show that the expression of multiple protein 4.1 polypeptides is regulated at the levels of translation and RNA processing.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blood Proteins/biosynthesis,genetics Chickens Cytoskeletal Proteins DNA/genetics Erythropoiesis Gene Expression Regulation Membrane Proteins Neuropeptides Protein Biosynthesis RNA Processing, Post-Transcriptional RNA, Messenger/biosynthesis,genetics
Chemicals
Blood Proteins Cytoskeletal Proteins Membrane Proteins Neuropeptides RNA, Messenger erythrocyte membrane band 4.1 protein erythrocyte membrane protein band 4.1-like 1 DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ngai J
Stack J H
Moon R T
Lazarides E
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.13.4432
Published
1987-07-00
Pages
4432-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC305103
Subset
IM
Grants
NIGMS NIH HHS · T32 GM07616 · United States
Databases
GENBANK
M16962
External Links
PubMed source DOI: 10.1073/pnas.84.13.4432 PMC Full Text View journal details
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