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PMID: 3477813 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Increased expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 19 ·1987-10-00 ·Pages 6899-903

Wong AJ, Bigner SH, Bigner DD, Kinzler KW, Hamilton SR, Vogelstein B

Abstract

Primary malignant gliomas from 63 patients were analyzed to determine the relationship between amplification of the gene encoding the epidermal growth factor receptor (EGFR) and expression of the corresponding mRNA. Twenty-four tumors were found to have amplified the EGFR gene and amplification of other genes occurred in three additional tumors. Hybridization with synthetic RNA probes was used to quantitate mRNA levels in situ. All 24 tumors with amplification of the EGFR gene had high levels of expression of this gene, while none of the 39 tumors without amplification had increased levels. This shows that, in human gliomas, large increases in the expression of the EGFR gene are invariably associated with alterations in gene structure.

MeSH Terms
ErbB Receptors/genetics Female Gene Amplification Genes Glioma/genetics,metabolism,pathology Humans Male Middle Aged Nucleic Acid Hybridization Oncogenes RNA, Messenger/analysis
Chemicals
RNA, Messenger ErbB Receptors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wong A J
Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Bigner S H
Bigner D D
Kinzler K W
Hamilton S R
Vogelstein B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.19.6899
Published
1987-10-00
Pages
6899-903
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299192
Subset
IM
Grants
NCI NIH HHS · CA-09243 · United States
NCI NIH HHS · CA-11998 · United States
NINDS NIH HHS · NS-20023 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.19.6899 PMC Full Text View journal details
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