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PMID: 3837853 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Deregulation of c-myc gene expression in human colon carcinoma is not accompanied by amplification or rearrangement of the gene.

Molecular and cellular biology ·Vol. 5 ·No. 8 ·1985-08-00 ·Pages 1969-76

Erisman MD, Rothberg PG, Diehl RE, Morse CC, Spandorfer JM, Astrin SM

Abstract

The structure and expression of the c-myc oncogene were examined in 29 primary human colon adenocarcinomas. Dot blot hybridization of total RNA showed that 21 tumors (72%) had considerably elevated expression of c-myc (5- to 40-fold) relative to normal colonic mucosa. These data were corroborated by Northern blots of polyadenylated RNA, which showed a 2.3-kilobase transcript. Southern analysis of the c-myc locus in these tumors indicated the absence of amplification or DNA rearrangement in a 35-kilobase region encompassing the gene. In a parallel study, elevated expression of c-myc without amplification or DNA rearrangement was also observed in three of six colon carcinoma cell lines examined; in addition, unlike a normal colon cell line control, these three cell lines exhibited constitutive, high-level expression of the gene during their growth in cultures. These results indicate that elevated expression of the c-myc oncogene occurs frequently in primary human colon carcinomas and that the mechanism involved in the regulation of c-myc expression is altered in tumor-derived cell lines.

MeSH Terms
Cell Line Cells, Cultured Colon/metabolism Colonic Neoplasms/genetics Gene Amplification Genes, Regulator Humans Nucleic Acid Hybridization Oncogenes RNA, Neoplasm/isolation & purification Transcription, Genetic
Chemicals
RNA, Neoplasm
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Erisman M D
Rothberg P G
Diehl R E
Morse C C
Spandorfer J M
Astrin S M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.8.1969-1976.1985
Published
1985-08-00
Pages
1969-76
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366914
Subset
IM
Grants
NCI NIH HHS · CA-09035 · United States
NCI NIH HHS · CA-27797 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.8.1969-1976.1985 PMC Full Text View journal details
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